胃溃疡是最常见的胃肠道疾病之一。本研究调查了硒（Se）和卵磷脂（Lec）单独及联合对乙醇诱导的小鼠胃溃疡的抗氧化和抗炎效应，以及它们调节胰岛素样生长因子-1（IGF-1）/丢失染色体10号磷酸酯酶（PTEN）/蛋白激酶B（Akt）/ Forkhead盒子O3a（FoxO3a）信号转导的能力。小鼠分为正常组、乙醇组、Se + 乙醇组、Lec + 乙醇组、Se + Lec + 乙醇组和奥美拉唑 + 乙醇组。选定剂量的治疗在给予小鼠单次绝对乙醇（5 ml/kg体重）诱导胃溃疡前连续进行14天。结果显示，Se和Lec联合预处理显著减轻了乙醇组的胃溃疡的巨观和微观病变，并增加了保护指数。值得注意的是，Se和Lec联合治疗降低了胃组织中的活性氧、丙二醛和细胞色素c水平，并增加了谷胱甘肽、谷胱甘肽过氧化物酶和硫氧还蛋白还原酶活性。Se和Lec联合治疗增加了前列腺素E2和白细胞介素-10水平，但降低了肿瘤坏死因子-α、白细胞介素-6和白细胞介素-1β水平，与单独治疗相比。有趣的是，这种联合治疗降低了胃组织中IGF-1、p-Akt和FoxO3a蛋白的表达，并增加了PTEN的表达。伽str蓝和嗪红染色的胃组织检查证实了这些结果。因此，与单独治疗相比，Se和Lec的联合治疗通过抗氧化和抗炎作用，在调节IGF-1/PTEN/Akt/FoxO3a途径信号转导方面对乙醇诱导的小鼠胃溃疡具有更优的保护作用。© 2023. 本文作者，Springer Science+Business Media, LLC和Springer Nature LLC独家许可。

Gastric ulcers are one of the most prevalent gastrointestinal disorders. The current study investigated the antioxidant and anti-inflammatory effects of selenium (Se) and lecithin (Lec) alone and in combination against ethanol-induced gastric ulcers in mice, and their ability to modulate insulin-like growth factor-1 (IGF-1)/ Phosphatase and tensin homologue deleted on chromosome 10 (PTEN)/ Protein kinase B (Akt)/ Forkhead box O3a (FoxO3a) signaling. The mice were divided into normal, ethanol, Se + ethanol, Lec + ethanol, Se + Lec + ethanol, and omeprazole + ethanol groups. Treatment with the selected doses was continued for 14 days before a single dose of absolute ethanol (5 ml/kg body weight) was administered to induce gastric ulcers in mice. The results showed that pretreatment with Se and Lec combination effectively decreased both the macro- and microscopic gastric lesions and increased the protection index compared to the ethanol group. Remarkably, the Se and Lec combination decreased the levels of reactive oxygen species, malondialdehyde, and cytochrome c and increased glutathione, glutathione peroxidase, and thioredoxin reductase activities in gastric tissues. The Se and Lec combination increased prostaglandin E2 and interleukin-10 levels but decreased tumor necrosis factor-α, interleukin-6 and interleukin-1β levels compared to either treatment alone. Interestingly, this combination decreased the expression of IGF-1, p-Akt, and FoxO3a proteins and increased PTEN expression in gastric tissues. The gastric tissues examination by hematoxylin and eosin staining confirmed these results. Therefore, the Se and Lec combination showed superior protective effects against ethanol-induced gastric ulcers in mice, compared to either treatment alone, through antioxidant, and anti-inflammatory activities, in addition to modulating IGF-1/PTEN/Akt/FoxO3a pathway signaling.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.