在这项工作中，我们采用了一种环境友好的策略，利用欧洲橄榄（橄榄）果实合成了金纳米颗粒（Au NPs）。采用透射电子显微镜（TEM）、紫外-可见光谱、X射线衍射（XRD）和能量色散X射线（EDX）对合成的纳米颗粒的物化性质进行表征。使用Au NPs修饰的玻碳电极研究了肼的直接电化学氧化反应。所提出的肼传感器具有良好的性能，如较宽的线性范围（2.5-275 μM）、低检测限（0.09 μM）、显著的选择性和优异的重现性（RSD = 2.2%）。还通过使用从橄榄果实提取的Au NPs的不同浓度来探索三种人类癌细胞系（KATOIII，NCI-N87和SNU-16）的体外细胞毒性。根据MTT试验方案，发现生物合成的Au NPs对人类胃癌具有细胞毒性。所得结果表明，绿色合成的Au NPs可以成功应用于电化学传感和癌症治疗应用。版权所有 © 2023. Elsevier Inc. 发布。

In this work, an environmentally friendly strategy was used to synthesize gold nanoparticles (Au NPs) using Olea europaea (olive) fruit. Transmission electron microscopy (TEM), UV-Vis spectroscopy, X-ray diffraction (XRD) and energy-dispersive X-ray (EDX) were used to characterize the physicochemical properties of the synthesized NPs. An Au NPs modified glassy carbon electrode was used to investigate the direct electrochemical oxidation of hydrazine. The suggested hydrazine sensor has good performance, such as a wide linear range (2.5-275 μM), low limit of detection (0.09 μM), notable selectivity and excellent reproducibility (RSD = 2.2%). The in-vitro cytotoxicity of three human cancer cell lines (KATOIII, NCI-N87, and SNU-16) was also explored with various concentrations of Au NPs prepared from olive fruit extract. Bio-synthesized Au NPs were found to have cytotoxic properties against gastric cancer in humans based on MTT assay protocol. The obtained results show that green synthesized Au NPs can be successfully employed in electrochemical sensing and cancer treatment applications.Copyright © 2023. Published by Elsevier Inc.