研究动态
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蓝光通过抑制水杨酸合成,增强西兰花(Brassica oleracea var. italica)幼苗中有益健康的硫代谢产物硫苷萝卜苷的积累。

Blue Light Enhances Health-Promoting Sulforaphane Accumulation in Broccoli (Brassica oleracea var. italica) Sprouts through Inhibiting Salicylic Acid Synthesis.

发表日期:2023 Sep 01
作者: Youyou Guo, Chunyan Gong, Beier Cao, Tiantian Di, Xinxin Xu, Jingran Dong, Keying Zhao, Kai Gao, Nana Su
来源: ANTIOXIDANTS & REDOX SIGNALING

摘要:

作为一种营养价值高的蔬菜,西兰花(Brassica oleracea var.italica)富含维生素、抗氧化剂和抗癌化合物。硫代葡萄糖苷(GLs)是十字花科蔬菜中广泛存在的重要功能成分之一,它们的水解物硫代异硫氰酸酯(SFN)在抗癌过程中起着关键作用。本研究揭示了蓝光显著诱导了西兰花萌芽体中SFN的含量,并且水杨酸(SA)参与了此过程。我们研究了蓝光处理下西兰花萌芽体中SFN积累的分子机制以及SFN与SA之间的关系。结果表明,蓝光照射显著增加了西兰花萌芽体中的SFN积累,并且SA在蓝光条件下特别上调了SFN合成相关基因的表达。此外,与白光相比,蓝光显著降低了SA的含量,并且这种降低受到了抑制SA合成的paclobutrazol(Pac)的更大抑制。另外,在蓝光处理下,SFN合成相关基因的转录水平和丙型芥子硫化酶(MYR)的活性与SFN积累趋势相一致。总的来说,我们得出结论:SA参与了蓝光条件下西兰花萌芽体中SFN的积累。
As a vegetable with high nutritional value, broccoli (Brassica oleracea var. italica) is rich in vitamins, antioxidants and anti-cancer compounds. Glucosinolates (GLs) are one of the important functional components widely found in cruciferous vegetables, and their hydrolysate sulforaphane (SFN) plays a key function in the anti-cancer process. Herein, we revealed that blue light significantly induced the SFN content in broccoli sprouts, and salicylic acid (SA) was involved in this process. We investigated the molecular mechanisms of SFN accumulation with blue light treatment in broccoli sprouts and the relationship between SFN and SA. The results showed that the SFN accumulation in broccoli sprouts was significantly increased under blue light illumination, and the expression of SFN synthesis-related genes was particularly up-regulated by SA under blue light. Moreover, blue light considerably decreased the SA content compared with white light, and this decrease was more suppressed by paclobutrazol (Pac, an inhibitor of SA synthesis). In addition, the transcript level of SFN synthesis-related genes and the activity of myrosinase (MYR) paralleled the trend of SFN accumulation under blue light treatment. Overall, we concluded that SA participates in the SFN accumulation in broccoli sprouts under blue light.