广藿香（PR）是一种宝贵的草药，广泛用于临床慢性阻塞性肺疾病的治疗。然而，慢性阻塞性肺疾病的治疗机制尚不清楚，因缺乏体内实验研究。本研究建立了一种基于超高效液相色谱联用飞行时间质谱、网络药理学和分子对接的新型综合策略，系统分析了PR在体内的组织分布和活性化合物，以及慢性阻塞性肺疾病的治疗机制。首先，组织分布研究表明肺是PR化合物分布最高的器官。随后，网络药理学结果显示，肿瘤坏死因子信号通路、白细胞介素-17信号通路和丝裂原活化蛋白激酶信号通路是PR对抗慢性阻塞性肺疾病的关键机制。最后，分子对接结果显示关键靶点与PR对应的活性化合物稳定结合。我们的研究对于筛选关键有效化合物和研究中药的作用机制具有重要意义，并为广藿香的进一步开发和利用提供了数据支持。©2023 Wiley-VCH GmbH.

Platycodi Radix (PR) is a valuable herb that is widely used in the treatment of chronic obstructive pulmonary disease in clinics. However, the mechanism of action for the treatment of chronic obstructive pulmonary disease remains unclear due to the lack of in vivo studies. Our study established a novel integrated strategy based on ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry, network pharmacology, and molecular docking to systematically analyze the tissue distribution and active compounds of PR in vivo and the therapeutic mechanism of chronic obstructive pulmonary disease. First, tissue distribution studies have shown that the lung is the organ with the highest distribution of PR compounds. Subsequently, network pharmacology results showed that the tumor necrosis factor signaling pathway, interleukin-17 signaling pathway, and mitogen-activated protein kinase signaling pathway were the critical mechanisms of PR against chronic obstructive pulmonary disease. Ultimately, molecular docking results showed that the key targets were stably bound to the corresponding active compounds of PR. Our study is of great significance for the screening of the key effective compounds and the study of the mechanism of action in traditional Chinese medicine and provides data to support the further development and utilization of PR.© 2023 Wiley-VCH GmbH.