本研究旨在探讨PD-L1（程序性细胞死亡配体-1）和PD-1（程序性细胞死亡蛋白-1）在结肠腺癌肿瘤新生中的表达关系。研究共纳入了122名结肠腺癌患者，分别选择最大的固定在福尔马林和石蜡中的肿瘤组织样本进行分析。根据围肿瘤和肿瘤内细胞百分比（0％，1％，1-5％，>5％），计算和分级了肿瘤组织中膜PD-L1（22C3克隆）和综合阳性分数（CPS）的表达情况。分析了这些因素对预后的影响。肿瘤新生与不良临床病理特征和差劲的总生存率相关。膜PD-L1（CPS%）围肿瘤新生（1％/<1％）在单变量模型中具有统计学意义（p = 0.004）。年龄、器官转移（肝脏、肺、膈肌、腹膜）在多变量模型中具有统计学意义（p = 0.001，p = 0.004，p = 0.001，p = 0.002，p = 0.004和p = 0.032，分别）。PD-L1阳性染色主要观察到在肿瘤周围和肿瘤新生期间。PD-L1围肿瘤新生率和患者的生存率存在显著差异（log-rank = 12.07，p = 0.007）。我们发现PD-L1（CPS%）> 1％的肿瘤新生患者具有较短的预期寿命，并说明了在进行生物标志物评估时包括肿瘤新生区域的重要性。我们先前报道了肿瘤新生中PD-L1表达与更具侵袭性的癌症生物学和较差的生存率相关，尽管总生存率具有有限的统计学意义。版权所有 © 2023 Elsevier Inc. 保留所有权利。

In this study, we investigated the relationship between programmed cell death ligand 1 (PD-L1) and programmed cell death protein 1 (PD-1) expression in colon adenocarcinoma tumor budding.This study included 122 patients with colon adenocarcinomas. The largest sample of formaldehyde-fixed paraffin-embedded tumor tissues was selected for analysis. Expression of membranous PD-L1 (clone 22C3) and the Combined Positive Score (CPS) in tumor tissues was calculated and graded according to the percentages of peritumoral and intratumoral tumor cells (0 %, 1 %, 1-5 %, >5 %). The effects of these factors on the prognosis were analyzed.Tumor budding was associated with adverse clinicopathological features and poor overall survival. PD-L1 (CPS%) peritumoral tumor budding (1 %/<1 %) was statistically significant in the univariate model (p = 0.004). Age, organ metastases (liver, lung, liver, lung, and peritoneum), and metastases were statistically significant in the multivariate model (p = 0.001, p = 0.004, p = 0.001, p = 0.002, p = 0.004, and p = 0.032, respectively). PD-L1 positive staining was mostly observed around the tumor and during tumor budding. PD-L1 peritumoral tumor budding rates and patients' survival rates differed significantly (log-rank = 12.07, p = 0.007).We found that patients with PD-L1 (CPS%) > 1 % in tumor budding had a shortened life expectancy and demonstrated the importance of including tumor budding areas in the samples used for biomarker evaluation. We previously reported that PD-L1 expression in tumor budding is associated with more aggressive cancer biology and poor survival, although overall survival is of limited statistical significance.Copyright © 2023 Elsevier Inc. All rights reserved.