基于纳米颗粒（NPs）介导的基因传感器电极在医学诊断方面得到了巨大的发展。本文报告了一种简便、快速、低成本且高灵敏度的生物传感策略，用于早期检测HPV 18，并利用沉积在微型互联电极（micro-IDEs）上的金纳米颗粒（AuNPs）。本研究表明，微细互联电极（micro-IDE）表面带电性传导的特性，用二氧化硅绝缘的氧化铝进行改性，独立和迷你基因传感器修饰的胶体金纳米颗粒（AuNPs），以及通过基因杂交来早期检测宫颈癌。AuNPs沉积的微型互联电极表面经过了最优化的3-氨基丙基三乙氧基硅烷（APTES）的修饰，随后与脱氧核糖核酸（DNA）病毒杂交，形成DNA基因传感器。单链DNA与人乳头状瘤病毒（HPV）18的单链DNA靶标的杂交结果表明，经过胶体AuNPs修饰的微型互联电极在0.529 aM的最低检测中起到了肯定作用。基于相关系数回归分析，经过AuNPs修饰的微型互联电极在灵敏度测试中取得了更好的结果（R2=0.99793），优于未经修饰的微型互联电极。版权所有 © 2023 Elsevier B.V. 发表。

Genosensor-based electrodes mediated with nanoparticles (NPs) have tremendously developed in medical diagnosis. Herein, we report a facile, rapid, low cost and highly sensitive biosensing strategy for early detection of HPV 18 using gold-nanoparticles (AuNPs) deposited on micro-IDEs. This study represents surface charge transduction of micro-interdigitated electrodes (micro-IDE) alumina insulated with silica, independent and mini genosensor modified with colloidal gold NPs (AuNPs), and determination of gene hybridization for early detection of cervical cancer. The surface of AuNPs deposited micro-IDE functionalized with optimized 3-aminopropyl-triethoxysilane (APTES) followed by hybridization with deoxyribonucleic acid (DNA) virus to develop DNA genosensor. The results of ssDNA hybridization with the ssDNA target of human papillomavirus (HPV) 18 have affirmed that micro-IDE functionalized with colloidal AuNPs resulted in the lowest detection at 0.529 aM. Based on coefficient regression, micro-IDE functionalized with AuNPs produces better results in the sensitivity test (R2 = 0.99793) than unfunctionalized micro-IDE.Copyright © 2023. Published by Elsevier B.V.