研究表明，鳄梨种子是一种良好的生物活性化合物来源。本研究调查了烘烤对发酵鳄梨种子代谢物和抗癌活性的影响。所有三种抗癌活性的发酵鳄梨种子在较低的烘烤温度和时间下均更高。对于Hep G2、MDA-MB-231和MCF-7癌细胞系，最佳抑制效果发生在Hep G2癌细胞系，其次是MDA-MB-231和MCF-7癌细胞系。利用气相色谱-质谱技术进行无目标代谢物分析，共鉴定了208个代谢物。共有41个代谢物通过PLS-R方法，其VIP值大于1，并且与抗癌活性有关。所有氨基酸和大多数糖类在较低的烘烤温度下含量较高，并且与抗癌活性呈正相关。确定了最佳抗氧化和抗癌活性的烘烤条件为121°C烘烤9分钟。研究结果显示，发酵鳄梨种子粉有潜力成为一种具有有益生物活性属性的功能性食品成分。版权所有 © 2023 Elsevier Ltd. 保留所有权利。

Studies have demonstrated avocado seeds are a good source of bioactive compounds. This study investigated the effects of roasting on the metabolites and anticancer activities of fermented avocado seeds. All three anti-cancer activities of fermented avocado seeds were higher at lower roasting temperature and time. The best inhibition effect was found against Hep G2 followed by the MDA-MB-231 and MCF-7 cancer cell lines. Untargeted metabolite profiling using gas chromatography-mass spectrometry resulted in identification of 208 metabolites. In total, 41 metabolites identified had VIP values more than 1 using PLS-R that were related to anticancer activities. All amino acids and most sugars were higher at lower roasting temperature and positively correlated to anticancer activity. The roasting conditions for optimal antioxidant and anticancer activities were determined to be 121 °C for 9 min. Findings showed that fermented avocado seed powder has the potential to become a functional food ingredient with beneficial bioctive properties.Copyright © 2023 Elsevier Ltd. All rights reserved.