影响干细胞治疗视网膜退化的因素。
Factors Affecting Stem Cell-Based Regenerative Approaches in Retinal Degeneration.
发表日期：2023 Sep 15
作者：
Sachin H Patel, Deepak A Lamba
来源：
Stem Cell Research & Therapy
摘要：
遗传性和与年龄相关的视力丧失通常与视网膜细胞退化有关，这些细胞位于眼睛后方的感光层。哺乳动物的视网膜是一个后分裂神经组织，无法通过内源性再生来修复自身。自从人类多能干细胞的分离和培养方法的发展以及诱导多能干细胞的生成出现以来，对细胞替代方法的开发引起了相当大的兴奋。现在，这种方法已与模拟体外人类视网膜发育的新型三维器官培养系统结合起来。在本综述中，我们重点介绍该领域的当前状态，包括细胞输送挑战、接受者免疫微环境的作用以及与移植细胞与宿主电路之间的局部和整体连接的挑战。
Inherited and age-associated vision loss is often associated with degeneration of the cells of the retina, the light-sensitive layer at the back of the eye. The mammalian retina, being a postmitotic neural tissue, does not have the capacity to repair itself through endogenous regeneration. There has been considerable excitement for the development of cell replacement approaches since the isolation and development of culture methods for human pluripotent stem cells, as well as the generation of induced pluripotent stem cells. This has now been combined with novel three-dimensional organoid culture systems that closely mimic human retinal development in vitro. In this review, we cover the current state of the field, with emphasis on the cell delivery challenges, role of the recipient immunological microenvironment, and challenges related to connectivity between transplanted cells and host circuitry both locally and centrally to the different areas of the brain.