为了探究体内不相干运动（IVIM）和扩散峰系数成像（DKI）参数与缺氧生物标志物（即缺氧诱导因子-1α（HIF-1α），碳酸酐酶IX（CAIX）和吡莫啶（PIMO））在纤维肉瘤（FS）小鼠模型中的相关性，建立了一个由30只FS裸鼠组成的模型。所有小鼠在行磁共振成像（MRI）扫描后，获取了IVIM（标准表观扩散系数[标准ADC]，纯扩散系数[D]，伪扩散系数[D*]和灌注分数[f]）和DKI参数（平均扩散[MD]，平均峰系数[MK]）。基于MRI-病理学受控方法，用Pearson或Spearman检验评估了每个MRI参数与缺氧生物标志物之间的相关性。采用独立样本t检验或Wilcoxon秩和检验以及受试者工作特征曲线来确定MRI参数是否能区分高和低表达的缺氧生物标志物。IVIM/DKI参数分别与HIF-1α，CAIX和PIMO表达呈不同程度的相关性。其中，D，f和MK值可以确认HIF-1α的表达，而D，f和MK值可以评估CAIX的表达。最后，标准D和MK值可以评估PIMO的表达水平。IVIM和DKI参数可以用来反映纤维肉瘤的缺氧生物标志物，并有潜力检测肿瘤缺氧。版权所有 © 2023 The Association of University Radiologists。由Elsevier Inc.出版。保留所有权利。

To investigate whether intravoxel incoherent motion (IVIM) and diffusion kurtosis imaging (DKI) parameters correlate with hypoxia biomarkers, namely hypoxia inducible factor-1ɑ (HIF-1ɑ), carbonic anhydrase IX (CAIX), and pimonidazole (PIMO), in fibrosarcoma (FS) murine models.A model of 30 FS nude mice was established. All mice underwent magnetic resonance imaging (MRI) scans after which the IVIM (standard apparent diffusion coefficient [standard ADC], pure diffusion coefficient [D], pseudo-diffusion coefficient [D*], and perfusion fraction [f]) and DKI parameters (mean diffusion [MD], mean kurtosis [MK]) were obtained. Based on an MRI-pathology controlled method, correlations between each MRI parameter and hypoxia biomarkers were assessed by Pearson or Spearman tests. An independent sample t-test or Wilcoxon's rank sum test, and receiver operating characteristic curves were used to identify whether MRI parameters could differentiate between high and low expressions of hypoxia biomarkers.The IVIM/DKI parameters showed varying degrees of correlation with HIF-1α, CAIX, and PIMO expression. Among them, the D, f, and MK values could confirm HIF-1α expression, while D, f, and MK values could assess CAIX expression. Finally, standard D and MK values could evaluate PIMO expression levels.IVIM and DKI parameters can be used to reflect hypoxic biomarkers of FS and have the potential to detect tumor hypoxia.Copyright © 2023 The Association of University Radiologists. Published by Elsevier Inc. All rights reserved.