为了研究炎症分子通过益肺清化颗粒和含酚醛的血清调节肿瘤微环境和抗肿瘤的作用机制，并干预体外肺癌细胞和骨髓细胞共培养中肿瘤微环境的变化。本研究选择了A549肺腺癌细胞系和ST2骨髓基质细胞系，并使用转移室制备了两种细胞共培养体系。它们被分为正常盐水组、酚醛组、益肺清化颗粒组和酚醛+益肺清化颗粒组，分别进行含药血清干预。作为对照，则分别使用培养的A549细胞和ST2细胞。采用流式细胞仪检测ST2细胞骨髓细胞中MDSC和Treg的比例。采用ELISA检测培养上清液中炎症因子的水平。采用Western blot检测A549和ST2细胞中炎症通路的表达。ST2细胞和A549细胞进行共培养，导致ST2细胞中MDSC和Treg的比例增加，培养上清液中部分炎症因子水平上升，ST2细胞中炎症通路的表达水平增加。然而，A549细胞中炎症通路的表达水平没有明显改变。而益肺清化颗粒和酚醛能够部分逆转这些变化，并且两者联合使用比单一药物更有效。ST2细胞和A549细胞共培养后，细胞向MDSC和Treg的转化加速。益肺清化颗粒和酚醛的联合使用可以重塑肿瘤微环境，防止该现象发生，减少炎症分泌并抑制肿瘤细胞生长。这可能与抑制TNF-α/IL-1-和NF-κB/STAT3炎症通路的表达有关。

To investigate the mechanism of action of inflammatory molecules regulating the tumor microenvironment and anti-tumor through Yifei Qinghua granules and phloroglucinol-containing serum intervening in the changes of tumor microenvironment in vitro in the co-culture of lung cancer cells and bone marrow cells.  A549 lung adenocarcinoma cell line and ST2 bone marrow stromal cell line were selected and a transwell chamber was used to establish the co-culture system of the two kinds of cells. They were divided into normal saline, phloroglucinol, Qifei Qinghua granule, and phloroglucinol + Yifei Qinghua granule groups. They were given drug-containing serum interventions respectively. A549 cells and ST2 cells cultured separately were used as control. Flow cytometry was used to detect the proportions of MDSCs and Tregs in bone marrow cells of ST2 cells. ELISA was used to detect the levels of inflammatory factors in the culture supernatant. Western blot was used to detect the expressions of inflammatory pathways in A549 and ST2 cells. ST2 cells and A549 cells were co-cultured. The ratio of MDSCs and Treg in ST2 cells was increased. The levels of some inflammatory factors in the culture supernatant were increased. The expression level of the inflammatory pathway in ST2 cells was increased. However, the expression level of the inflammatory pathway in A549 cells had no obvious change. While Yifei Qinghua granule and phloroglucinol could partially reverse these changes. The combination of the two was more effective than a single drug. The conversion of cells to MDSCs and Treg was accelerated after the co-culture of ST2 cells and A549 cells. The combination of Yifei Qinghua granules with phloroglucinol can reshape the tumor microenvironment, prevent this phenomenon from occurring, reduce inflammatory secretion and inhibit tumor cell growth. This may be related to the inhibition of the expressions of TNF-α/IL-1- and NF-κB/STAT3 inflammatory pathways.