柚皮素调节喉咽鳞状细胞癌的侵袭、迁移和转移。
Nobiletin regulates invasion, migration and metastasis in hypopharyngeal squamous cell carcinoma.
发表日期：2023 Jul 31
作者：
Marisol Rosas-Martínez, José Antonio González Rosales, Gloria Gutiérrez-Venegas
来源：
Cellular & Molecular Immunology
摘要：
头颈部鳞状细胞癌预后不良。患者通常在确诊时呈晚期疾病，并表现出严重营养不良。Nobiletin（NOB）（5,6,7,8,3',4'-Hexamethoxyflavone）是一种多甲氧基黄酮，可抑制多种癌细胞类型的增殖和迁移。据我们所知，它对喉咽鳞状细胞癌的影响尚未进行评估。本研究考察了NOB对来源于喉咽鳞状细胞癌的FaDu细胞系的增殖、迁移和侵袭性的影响。我们通过Western blot分析蛋白激酶磷酸化、Transwell分析迁移以及酶联免疫吸附分析血管内皮生长因子的转移潜力来确定。NOB在浓度为15µM和40µM时抑制了细胞增殖40%，并在50µM时降低了迁移并诱导凋亡，通过下调外周信号调节激酶、蛋白激酶B和磷酸肌醇3-激酶来实现。我们的结果表明，NOB对FaDu细胞的影响与蛋白激酶抑制相关。
Head and neck squamous cell carcinoma carries a poor prognosis. Patients typically present with advanced disease and exhibit severe malnutrition at the time of diagnosis. Nobiletin (NOB) (5,6,7,8,3',4'-Hexamethoxyflavone) is a polymethoxyflavone that inhibits the proliferation and migration of various cancer cell types. To the best of our knowledge, its effects on hypopharyngeal squamous cell carcinoma have not been evaluated previously. This study examined the effects of NOB on the proliferation, migration, and invasiveness of the FaDu cell line derived from hypopharyngeal squamous cell carcinoma. We determined protein kinase phosphorylation by western blot analysis, migration by Transwell assay, and metastatic potential by enzyme-linked immunosorbent assay of vascular endothelial growth factor. NOB inhibited cell proliferation by 40% at concentrations of 15 µM and 40 µM, and reduced migration and induced apoptosis at 50 µM by downregulating extracellular signal-regulated kinase, protein kinase B, and phosphoinositide 3-kinase. Our results suggest that the effects of NOB on FaDu cells are associated with protein kinase inhibition.