为了比较低级和高级枕上脑胶质瘤之间与克氏循环相关的调控酶的组织水平，评估了40名接受枕上脑胶质瘤手术的患者（19例低级别和21例高级别胶质瘤）。使用ELISA方法对参与克氏循环的调控酶（包括丙酮酸脱氢酶、柠檬酸合成酶、α-酮戊二酸脱氢酶和异柠檬酸脱氢酶）和间接调控克氏循环的两种酶（谷氨酸脱氢酶和谷氨酰胺酶）在肿瘤组织中进行定量研究。将结果在两组之间进行比较。发现高级别胶质瘤组的所有酶水平均较高，但只有丙酮酸脱氢酶、柠檬酸合成酶和异柠檬酸脱氢酶的水平显著。此外，所有酶在二级别别与一级别别的胶质瘤中的组织水平都较高，但只有谷氨酸脱氢酶和谷氨酰胺酶的水平达到显著高水平。在高级别胶质瘤组中，所有酶在四级别别与三级别别胶质瘤中的组织水平再次较高，但没有达到统计学意义。克氏循环的调控酶在高级别胶质瘤中较低级别胶质瘤中水平增加。为了满足高级别胶质瘤的高能量需求，克氏循环所需的谷氨酸代谢酶谷氨酸脱氢酶和谷氨酰胺酶也增加。

To compare tissue levels of the regulatory enzymes related to the Krebs cycle between low, and high-grade supratentorial gliomas.Forty patients who underwent surgery for supratentorial gliomas (19 with low-grade and 21 with high-grade gliomas) were evaluated. The regulatory enzymes directly involved in the Krebs cycle, namely pyruvate dehydrogenase, citrate synthase, ?-ketoglutarate dehydrogenase, and isocitrate dehydrogenase, and two enzymes that indirectly regulate the Krebs cycle, namely glutamate dehydrogenase and glutaminase, were quantitatively studied in tumor tissues using ELISA. The results were compared between the two groups.The levels of all enzymes were higher in the high-grade glioma group but only pyruvate dehydrogenase, citrate synthase, and isocitrate dehydrogenase levels showed statistical significance. Moreover, all enzymes showed higher tissue levels in grade- II compared to grade-I gliomas, but only two enzymes, glutamate dehydrogenase and glutaminase, reached significantly higher levels. In the high-grade glioma group, all enzymes again showed higher tissue levels in grade-IV gliomas than in grade-III gliomas, but none showed statistical significance.Regulatory enzymes of the Krebs cycle are increased in high-grade gliomas compared to low-grade gliomas. Glutaminolysis enzymes, namely glutamate dehydrogenase and glutaminase, which are required for resupplying the Krebs cycle, are also increased in order to meet the high energy demand in high-grade gliomas.