光动力疗法（PDT）利用光敏剂是一种微创的恶性肿瘤治疗方法。然而，理想的光敏剂尚未确立。最近，我们开发了一种新的光敏剂，即葡萄糖结合的叶绿素e6（G-Ce6）。为了评估利用G-Ce6进行短药光间隔治疗（VTP）的血管靶向PDT的临床疗效，治疗犬自发发生的肿瘤。对五只患有自发发生的肿瘤的犬进行VTP的治疗。这些犬分别患恶性黑色素瘤（三例）、血管外皮细胞瘤（两例）和鳞状细胞癌（一例）。在激光照射之前，这些犬静脉注射了1-3 mg/kg的G-Ce6。使用677纳米二极激光器对肿瘤进行浅表或间质照射。多次进行VTP能够减小肿瘤大小，使三只犬完全缓解。观察到诸如正常组织周围水肿和瘘管等并发症，但水肿是自限的。瘘管经过VTP后形成的坏死组织清除后痊愈。我们的研究结果表明，使用G-Ce6进行VTP对于患有自发发生肿瘤的犬具有抗肿瘤效果。© 2023 The Authors. Veterinary Medicine and Science由约翰·威立出版社出版。

Photodynamic therapy (PDT) using photosensitisers is a minimally invasive treatment for malignant tumours. However, ideal photosensitisers are not yet established. Recently, we developed a new photosensitiser, glucose-conjugated chlorin e6 (G-Ce6).To evaluate the clinical efficacy of vascular-targeted PDT (VTP), a type of PDT utilising a short drug-light interval, using G-Ce6 to treat spontaneously occurring tumours in dogs.Five dogs with spontaneously occurring tumours (malignant melanoma: three; haemangiopericytoma: two; and squamous cell carcinoma: one) were subjected to VTP. These dogs were intravenously injected with G-Ce6 at doses of 1-3 mg/kg 5 min before laser irradiation. Tumours were superficially or interstitially irradiated using a 677-nm diode laser.Repeated VTP decreased tumour size, yielding complete remission in three dogs. Complications such as oedema surrounding normal tissues and fistulae were observed, and the oedema was self-limiting. The fistula was cured by debriding the necrotic tissues formed after VTP.Our findings demonstrate that VTP using G-Ce6 had antitumour effects in dogs with spontaneously occurring tumours.© 2023 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.