当前，来自手机、无线路由器、基站和其他来源的射频电磁场（RF-EMF）在我们的生活环境中无处不在，并且剂量正在增加。据报道，RF-EMF已被证实具有细胞毒性，并被认为是各种人类疾病的危险因素，因此需要更多的关注。近年来，利用线粒体钙传入蛋白（MCU）抑制剂干预线粒体钙摄取被认为是线粒体钙超载疾病（如神经退行性疾病、缺血/再灌注损伤和癌症）的潜在临床治疗方法，但此方法是否会增加RF-EMF暴露的健康风险尚不明确。为了解决我们的关切，我们进行了初步研究，确定抑制MCU是否会增加细胞对RF-EMF暴露的基因毒性，并发现短时（15分钟）暴露于1800 MHz RF-EMF显著诱导小鼠胚胎成纤维细胞（MEFs）中使用Ruthenium 360（Ru360），一种特异性MCU抑制剂处理时的DNA损伤和细胞凋亡，但对细胞周期、细胞增殖或细胞存活没有显著影响。总之，我们的结果表明，抑制MCU会增加RF-EMF暴露的基因毒性，需要更多关注这些治疗方法下RF-EMF暴露可能的健康影响。版权所有©2023作者。由Elsevier Inc.出版。保留所有权利。

Today, the existence of radio-frequency electromagnetic fields (RF-EMF) emitted from cell phones, wireless routers, base stations, and other sources are everywhere around our living environment, and the dose is increasing. RF-EMF have been reported to be cytotoxic and supposed to be a risk factor for various human diseases, thus, more attention is necessary. In recent years, interfere with mitochondrial calcium uptake by using mitochondrial calcium uniporter (MCU) inhibitor were suggested to be potential clinical treatment in mitochondrial calcium overload diseases, like neurodegeneration, ischemia/reperfusion injury, and cancer, but whether this approach increases the health risk of RF-EMF exposure are unknown. To address our concern, we did a preliminary study to determine whether inhibition of MCU will increase the genotoxicity of RF-EMF exposure in cells, and found that short-time (15 min) exposure to 1800 MHz RF-EMF induced significant DNA damage and cell apoptosis in mouse embryonic fibroblasts (MEFs) treated with Ruthenium 360 (Ru360), a specific inhibitor of MCU, but no significant effects on cell cycle, cell proliferation, or cell viability were observed. In conclusion, our results indicated that inhibiting MCU increases the genotoxicity of RF-EMF exposure, and more attention needs to be paid to the possible health impact of RF-EMF exposure under these treatments.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.