目前，臭氧水在抗菌和抗病毒方面被广泛应用，且未有任何安全问题的报道。因此，臭氧水可能对治疗葡萄球菌相关的皮肤病，如特应性皮炎（AD）和化脓性皮肤病具有临床应用的潜力。本研究旨在验证不同浓度（3和11 mg / L）的臭氧水对葡萄球菌种类的杀菌效果，以及评估臭氧水在AD和化脓性皮肤病小鼠模型中的抗炎作用。首先，通过对金黄色葡萄球菌和耐甲氧西林的S. pseudintermedius进行多种浓度的臭氧水的杀菌性质验证，发现3和11 mg / L的臭氧水在100倍以下稀释时对葡萄球菌具有显著杀菌效果。接下来，我们检测了臭氧水预处理的S. pseudintermedius对细胞毒性和细胞因子产生（白细胞介素（IL）-6和IL-8）的影响，并发现经臭氧水预处理的葡萄球菌引起的细胞毒性和细胞因子产生均显著抑制。体内实验显示，经臭氧水预处理的S. pseudintermedius显著抑制了小鼠化脓性皮肤病的发展；然而，在治疗方面观察到的效果有限。有趣的是，在AD小鼠模型中，3和11 mg / L的臭氧水表现出双重杀菌和抗炎效果。这一观察结果通过对干扰素-γ /肿瘤坏死因子刺激的人表皮角质形成细胞在体外接触臭氧时的细胞因子产生的显著抑制得到证实。这些发现表明，给予臭氧可以是治疗过敏性皮肤病，如AD的一种新颖的治疗方法。版权所有©2023 Elsevier B.V. 保留所有权利。

Currently, ozone water is utilized for antibacterial and antiviral purposes without any reported safety concerns. Therefore, ozone water may have clinical applications in treating staphylococcal-specific cutaneous diseases, such as atopic dermatitis (AD) and pyoderma. This study aimed to verify the bactericidal effects of ozone water at different concentrations (3 and 11 mg/L) against staphylococcal species in vitro, as well as evaluate the anti-inflammatory effects of ozone water in a mouse model of AD and pyoderma. Initially, the bactericidal properties of several concentrations of ozone water were confirmed with Staphylococcus aureus and methicillin-resistant S. pseudintermedius. Both 3 and 11 mg/L of ozone water exhibited a significant bactericidal effect against staphylococci at less than 100 times dilution. We next examined the cellular cytotoxicity and cytokine production (Interleukin (IL)-6 and IL-8) induced by S. pseudintermedius pre-treated with ozone water, and our findings indicated that cytotoxicity and cytokine production induced by staphylococci were significantly inhibited after ozone water pre-treatment. In vivo experiments showed that ozone water-pre-treated S. pseudintermedius significantly inhibited the development of pyoderma in mice; however, limited effects were observed in a therapeutic setting. Interestingly, ozone water at concentrations of 3 and 11 mg/L exhibits dual bactericidal and anti-inflammatory effects in mice with AD. This observation was corroborated by the significant inhibition of cytokine production in interferon-γ/tumor necrosis factor-stimulated human epidermal keratinocyte cells exposed to ozone in vitro. These findings indicate that administering ozone can be a novel therapeutic approach for managing allergic skin diseases, such as AD.Copyright © 2023 Elsevier B.V. All rights reserved.