骨骼中的微小肿瘤扩散超出肉眼可见的肿瘤部位，在外科肿瘤学中代表着一个重大风险，因为股骨切除边缘的空间复杂性无法通过快速骨骼分析技术来解决。近期，利用激光诱导击穿光谱法（LIBS）已被引入作为快速骨骼分析的一种有前途的选择。本研究旨在使用基于电解质紊乱追踪的LIBS深度剖析来评估骨骼中微小肿瘤扩散的检测。 在扩大切除术后，通过LIBS对病患段性下颌切除标本中受肿瘤浸润的下颌部分进行原生态研究。在健康骨骼和肿瘤边界的每个激光点位置上，分析了30个激光射击的光谱和电解质深度剖面。为了验证激光位置的组织学，下颌部分被用薄分割盘标记。 利用LIBS可以可靠地区分羟基磷灰石中的固态钙（Ca）和溶解钙中的可溶性钙，并能反映健康骨骼的自然异质性。除了典型健康骨骼光谱外，增加的钾（K）发射值是骨骼肿瘤浸润的首个光谱征象。基于K分布的LIBS深度剖析可以用于追踪骨骼中与肿瘤相关的变化，并且具有激光射击准确性的肿瘤边界区域。 使用LIBS进行深度剖析可能有助于检测骨骼中的微小肿瘤扩散。未来，应将基于LIBS的直接电解质追踪应用于外科肿瘤学中的其他术中挑战，以通过光谱光学技术推进快速骨骼分析。 ©2023. 作者。

Microscopic tumor spread beyond the macroscopically visible tumor mass in bone represents a major risk in surgical oncology, where the spatial complexity of bony resection margins cannot be countered with rapid bone analysis techniques. Laser-induced breakdown spectroscopy (LIBS) has recently been introduced as a promising option for rapid bone analysis. The present study aimed to use LIBS-based depth profiling based on electrolyte disturbance tracking to evaluate the detection of microscopic tumor spread in bone.After en bloc resection, the tumor-infiltrated mandible section of a patient's segmental mandibulectomy specimen was natively investigated using LIBS. Spectral and electrolytic depth profiles were analyzed across 30 laser shots per laser spot position in healthy bone and at the tumor border. For the histological validation of the lasered positions, the mandibular section was marked with a thin separating disc.Solid calcium (Ca) from hydroxyapatite and soluble Ca from dissolved Ca can be reliably differentiated using LIBS and reflect the natural heterogeneity of healthy bone. Increased potassium (K) emission values in otherwise typically healthy bone spectra are the first spectral signs of tumorous bone invasion. LIBS-based depth profiles at the tumor border region can be used to track tumor-associated changes within the bone with shot accuracy based on the distribution of K.Depth profiling using LIBS might enable the detection of microscopic tumor spread in bone. In the future, direct electrolyte tracking using LIBS should be applied to other intraoperative challenges in surgical oncology to advance rapid bone analysis by spectroscopic-optical techniques.© 2023. The Author(s).