本研究采用原位剥离的天然多糖Cordia myxa (CMX) 促进了氧化锌纳米颗粒对p-硝基苯酚(p-NP)的生态友好催化氢化和微生物生长抑制的利用。多糖介导的生物合成纳米复合材料具有廉价、环保、绿色等特点。本研究利用CMX胶作为生物还原剂制备多功能、环境友好的氧化锌纳米复合材料（ZnO NPs）。该过程具有较低的反应时间和温度，并利用CMX作为还原剂和稳定剂。对CMX-ZnO纳米复合材料进行了结构、形态和光学性质的表征。生物合成的CMX-ZnO NPs表现出强大的催化活性和循环利用能力，可迅速氧化有害的p-NPs。在过量的NaBH4存在下，4-NP完全还原为CMX-ZnO NPs的时间为15分钟，具有再生能力和伪一阶动力学，其速率常数为0.2571 min-1。此外，人类结肠癌（HCT116）和3T3L1细胞株对ZnO纳米颗粒的细胞毒性作用非常敏感。基于碟片扩散法测得的抑菌圈直径，CMX-ZnO NPs对人类致病的革兰阳性和革兰阴性细菌（Bacillus，Salmonella，E. coli 和Pseudomonas aeruginosa）表现出强大的抗菌活性。CMX-ZnO NPs的显著抗菌活性可以克服目前存在的长期环境可持续性处理水溶性有机污染物和微生物污染物的限制。版权所有 © 2023. Elsevier B.V.发布。

In situ exfoliated natural polysaccharide Cordia myxa (CMX) is used to promote the utilization of zinc-oxide nanoparticles for eco-friendly catalytic hydrogenation of p-nitrophenol (p-NP) and microbial growth inhibition. Polysaccharide-mediated biosynthetic nanocomposite materials are interesting because they are cheap, green, and environmentally friendly. This study uses CMX gum as a bioreduction to produce multifunctional, environmentally friendly zinc-oxide nanocomposites (ZnO NPs). The process involves a low reaction time and temperature and utilizes CMX as a reducing and stabilizing agent. The structural, morphological, and optical properties of the CMX-ZnO nanocomposite were characterized. The biosynthetic CMX-ZnO NPs exhibited robust catalytic activity and recycling capacity for rapidly oxidizing hazardous p-NPs. The complete reduction of 4-NP to CMX-ZnO NPs in excess NaBH4 was achieved within 15 min, with recyclability and pseudo-first-order kinetics with a rate constant of 0.2571 min-1. Additionally, human colon cancer (HCT116) and 3T3L1 cell lines were remarkably sensitive to the cytotoxic effects of ZnO nanoparticles. CMX-ZnO NPs exhibited potent antibacterial properties against human pathogenic gram-positive and gram-negative bacteria (Bacillus, Salmonella, E. coli, and Pseudomonas aeruginosa) based on the zone of inhibition measured by the disc-diffusion method. The significant antibacterial activity of CMX-ZnO NPs can overcome the current limitations associated with removing water-soluble organic pollutants and microbiological contaminants for long-term environmental sustainability.Copyright © 2023. Published by Elsevier B.V.