我们报道了以姜黄素诱导的金纳米棒作为一种光学传感平台，通过其自组装实现对特异性DNA靶标的检测。环境友好型还原剂（即姜黄素）和硝酸银在碱性介质（即pH 10）中的协同作用被认为是形成小型金纳米棒（AuNRs）的原因，其大致长度和直径分别为19.7±0.8 nm和6.0±0.5 nm，且具有较低的纵向表面等离子体共振（SPR），能够检测和分析不同的生物标志物。此外，为了评估合成的AuNRs的细胞摄取情况，我们分别对A549细胞和HEPG2细胞系进行了3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴化物（MTT）试验进行了细胞毒性研究，并显示出大致相似的细胞毒性。有趣的是，合成的具有光学和电子活性的AuNRs基纳米生物传感平台能够以低检测限（即LOD 8.6±0.15 pM）检测到特异性DNA靶标，具有较高的灵敏度和更好的选择性。最后，本研究提出了一种简化的生物介导方法，用于调控AuNRs的形状和尺寸，为与癌细胞相关的特异性DNA检测提供了敏感、选择性和可靠的纳米生物传感平台。版权所有© 2023 Elsevier B.V. 保留所有权利。

We are reporting curcumin-induced gold nanorods as an optical sensing platform for the detection of sequence-specific DNA target through their self-assembly. The combined effect of eco-friendly reducing agent (i.e., curcumin) and silver nitrate in a basic medium (i.e., pH 10) has been attributed for the formation of small gold nanorods (AuNRs) having approximate length and diameter i.e., 19.7 ± 0.8 nm and 6.0 ± 0.5 nm, respectively, and lower longitudinal surface plasmon resonance (SPR) enable to detect and analyse different biomarkers. Further, for evaluating cellular uptake of as-synthesized AuNRs, the cytotoxicity study has been carried out by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on A549 cells and HEPG2 cell lines, respectively, and shown approximately similar cytotoxicity. Interestingly, as-synthesized optically and electronically active AuNRs based nanobiosensing platform enable to detect sequence-specific DNA targets with low level of detection limit i.e., LOD 8.6 ± 0.15 pM for complimentary target (CT) DNA with higher sensitivity and better selectivity. Finally, this study is suggesting a simplistic bio-mediated approach of tuning the shape and size of AuNRs for sensitive, selective and reliable nanobiosensing platform for sequence-specific DNA detection related to cancer cells.Copyright © 2023 Elsevier B.V. All rights reserved.