EBV相关胃癌(EBVaGC)是一种常见的与EBV感染相关的恶性肿瘤。胰岛素样生长因子2(IGF2)是一种印迹基因，是调节生长的关键蛋白，特别是在正常胎儿发育期间。印迹失功能(LOI)是多种人类癌症中常见的表观遗传异常。然而，IGF2基因在胃癌中的启动子甲基化、印迹状态和功能尚不清楚。为了探索IGF2在胃癌发生和发展中的作用，我们通过Transwell、创伤愈合、CCK-8和流式细胞术等试验研究了IGF2在胃癌中的生物学功能。利用PCR-RFLP和BGS检测了胃癌组织中IGF2基因的印迹状态和启动子甲基化。结果显示，IGF2在胃癌组织中的表达较相邻组织高。EBVaGC组织中IGF2基因启动子甲基化和LOI显著高于EBV阴性胃癌(EBVnGC)组织。胃癌中IGF2的高表达可以促进胃癌细胞的迁移和增殖。我们的数据表明IGF2参与了胃癌的发生和发展。针对IGF2可能是胃癌的一个潜在治疗靶点。

Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) is a common malignant tumor associated with EBV infection. Insulin-like growth factor 2 (IGF2) is an imprinted gene and a key protein that regulates growth, especially during normal fetal development. Loss of imprinting (LOI), is a common epigenetic anomaly in a variety of human cancers. However, the promoter methylation, imprinting status and function of IGF2 gene in GC are unclear.To explore the role of IGF2 in the occurrence and development of gastric cancer.The biological function of IGF2 in gastric cancer was investigated by Transwell, wound healing, CCK-8 and flow cytometry assays. IGF2 imprinting status and gene promoter methylation in gastric cancer tissues were detected by PCR-RFLP and BGS.The results showed that the expression of IGF2 was higher in GC tissues than adjacent tissues. IGF2 gene promoter methylation and LOI were significantly higher in EBVaGC tissues than in EBV-negative gastric cancer (EBVnGC) tissues. The high expression of IGF2 in gastric cancer can promote the migration and proliferation of gastric cancer cells.Our data suggest that IGF2 is involved in the occurrence and development of gastric cancer. Targeting IGF2 may be a potential therapeutic target for gastric cancer.