鉴于化疗药物对肺癌的严重副作用，以及磁场具有抗肿瘤特性和高安全性，本研究将中等或超高强度静态磁场 (SMFs) 与板蓝根甙 D (PD) 结合起来，探究其抗肿瘤效能和生物安全性。比较了 PD 与中等和超高 SMFs 与其无关联对携带 A549 细胞的小鼠的抗肿瘤效果。检测了小鼠体重、进食/水摄入量、血液常规、血液生化学、肿瘤重量以及组织苏木精和伊红 (H&E) 染色。采用提升式十字迷宫、开放式活动场和生命体征测试衡量行为。使用 RNA 测序 (RNA-seq) 检测 PD 与 SMFs 的结合靶点。结果显示，22 特斯拉 (T) 的 SMF 组的抗肿瘤效果是 2 mg/kg PD 组的 3.6 倍 (肿瘤生长抑制=10.08%)，而 150 毫特斯拉 (mT) SMF 的抗肿瘤效果只比 PD 组高 1.56 倍。尽管 PD 导致食物摄入量减少，但 PD 和 SMF 组之间的体重、水摄入量或食物摄入量没有显著差异。行为结果表明，PD 改善了小鼠的痛苦感，但 SMFs 降低了该效果。然而，在常规血液、血液生化学检测、H&E 染色或器官指数中，并未发现有显著异常，除了 PD 与 SMF 连用组的肾指数降低。RNA-seq 显示，SMFs 和 PD 协同靶向与肿瘤生长、炎症和神经疾病相关的基因表达。本研究显示了中等或超高 SMF 与 PD 结合治疗肺癌的抗肿瘤效果和生物安全性，只出现了少量的副作用，支持进一步研究磁场在临床应用中的可能性。版权所有：杨等。

Due to the serious side effects of chemotherapy drugs against lung cancer, and the antitumor properties and high safety of magnetic fields, the present study combined moderate or ultra-high intensity statics magnetic fields (SMFs) with platycodin D (PD) to explore the antitumor efficiency and biosafety. The antitumor effects of PD with or without moderate and ultra-high SMFs on A549 cells bearing mice were compared. Mouse body weight, food/water intake, hematology routine, blood biochemistry, tumor weight and tissues hematoxylin and eosin (H&E) staining were examined. Behavior was measured using the elevated plus maze, open field and vital signs tests. The combined targets of PD and SMFs were detected using RNA-sequencing (RNA-seq). The results showed that the antitumor effect of 22 Tesla (T) SMF group was 3.6-fold higher compared with that of the 2 mg/kg PD group (tumor growth inhibition=10.08%), while the antitumor effect of 150 mT SMF was only 1.56-fold higher compared with that of PD. Although PD reduced the food intake, there was no significant difference in body weight, water intake or food consumption among PD and SMF groups. Behavioral results indicated that PD ameliorated dysphoria in mice, but SMFs reduced this effect. However, no significant abnormalities were found in routine blood, blood biochemistry test, H&E staining or organ index, except renal index which was reduced by PD with or without SMFs. RNA-sequencing (RNA-seq) demonstrated that SMFs and PD synergistically targeted the expression of genes associated with tumor growth, inflammation and neurological disease. The present study showed the antitumor efficacy and biosafety of moderate or ultra-high SMF combined with PD, which exhibited only few side effects in the treatment of lung cancer, thus supporting further research for the clinical application of magnetic fields.Copyright: © Yang et al.