跨膜4 L6家族成员1（TM4SF1）和圆盘结构域受体1（DDR1）在许多类型的癌症中都有表达，但它们在上皮性卵巢癌中的表达情况以及其与患者预后的关系尚不清楚。本研究旨在探讨TM4SF1和DDR1在上皮性卵巢癌中的表达情况以及它们与预后的关系。首先，使用Oncomine和基因表达谱分析交互式分析（GEPIA）平台比较了TM4SF1和DDR1在卵巢癌和正常卵巢组织中的表达水平，使用Kaplan-Meier plotter分析了基因表达与患者预后之间的关系。使用Search Tool for the Retrieval of Interacting Genes/Proteins（STRING）分析与TM4SF1和DDR1相互作用的蛋白质，并对相互作用蛋白质进行基因本体论富集分析和Kyoto Encyclopedia of Genes and Genomes途径分析。此外，进行了免疫组化染色以检测上皮性卵巢癌组织中TM4SF1和DDR1蛋白的表达，并分析表达与预后之间的关系。Oncomine和GEPIA分析显示，TM4SF1和DDR1的表达水平在上皮性卵巢癌中明显高于正常卵巢组织，并且临床样本的分析揭示了TM4SF1和DDR1在某些病例中的共表达。STRING分析表明，TM4SF1和DDR1蛋白相互作用。上皮性卵巢癌中同时表达TM4SF1和DDR1的患者的总生存期和无进展生存期显著短于缺乏TM4SF1和DDR1共表达的患者。多变量分析表明，TM4SF1和DDR1蛋白的共表达是一个独立的预后因子。总之，TM4SF1和DDR1蛋白在一些上皮性卵巢癌组织中共表达，可能是上皮性卵巢癌的不良预后因子。此外，TM4SF1和DDR1可能具有相互作用或相互调控的机制。 版权：©Huang等人。

Transmembrane 4 L6 family member 1 (TM4SF1) and discoidin domain receptor 1 (DDR1) are expressed in numerous types of cancer, but their expression in epithelial ovarian cancer and the association between their expression and patient prognosis are unclear. The present study aimed to explore the expression of TM4SF1 and DDR1 and their relationship with prognosis in epithelial ovarian cancer. Firstly, the Oncomine and Gene Expression Profiling Interactive Analysis (GEPIA) platforms were used to compare the expression levels of TM4SF1 and DDR1 in ovarian cancer and normal ovarian tissue, and Kaplan-Meier plotter was used to analyze the association between gene expression and patient prognosis. The proteins interacting with TM4SF1 and DDR1 were analyzed using Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), and enrichment analysis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways was conducted for the interacting proteins. Furthermore, immunohistochemical staining was performed to detect the expression of TM4SF1 and DDR1 protein in epithelial ovarian cancer tissue and to analyze the association between expression and prognosis. The Oncomine and GEPIA analyses showed that the expression levels of TM4SF1 and DDR1 were significantly higher in epithelial ovarian cancer than in normal ovarian tissue, and the analysis of clinical samples revealed that TM4SF1 and DDR1 were coexpressed in some cases. STRING analysis indicated that the TM4SF1 and DDR1 proteins interact with each other. The overall survival and progression-free survival of patients whose epithelial ovarian cancer coexpressed TM4SF1 and DDR1 were significantly shorter than those of patients lacking TM4SF1 and DDR1 coexpression. Multivariate analysis indicated that TM4SF1 and DDR1 protein coexpression was an independent prognostic factor. In summary, TM4SF1 and DDR1 proteins were coexpressed in some epithelial ovarian cancer tissues and appear to be adverse prognostic factors for epithelial ovarian cancer. In addition, TM4SF1 and DDR1 may have an interactive or mutual regulatory mechanism.Copyright: © Huang et al.