由不同植物产生的环肽是一种很吸引人的研究领域，因为它们在生物活性和药物设计应用方面具有特殊的性质。环肽作为从自然界中获得新的生物活性的重要性引起了研究人员开发新的分离系统的兴趣。与常见的传统方法相比，基于芯片技术的分离和生物测定，尤其是用于抗癌治疗，具有闪亮的优势。本研究通过改进的微流控芯片对从Viola ignobilis中提取的Vigno 1-5环肽进行了极性和非极性力的液液相互作用分离，并将结果与传统的高效液相色谱（HPLC）技术进行了比较。传统的从植物中分离环肽的过程是一种昂贵且耗时的过程。本研究的科学创新之处在于利用改进的微流控芯片加速环肽的分离，具有低成本和高效率。结果表明，一种新颖且简单的微流控芯片概念是分离紫罗兰提取物中的Vigno群的一种有效方法。我们相信该概念可能被用于进一步的药物开发过程，特别是与传统的离线方法相比，通过在线生物测定芯片的结合来降低时间和成本。©TÜBİTAK.

Cyclotides as a cyclic peptide produced by different groups of plants have been a very attractive field of research due to their exceptional properties in biological activities and drug design applications. The importance of cyclotides as new biological activities from nature caused to attract researchers to develop new separation systems. Recent growth and development on chip-based technology for separation and bioassay especially for anticancer having sparklingly advantages comparison with common traditional methods. In this study, the microfluidic separation of Vigno 1-5 cyclotides extracted from Viola ignobilis by using polar and nonpolar forces as a liquid-liquid interaction was investigated through modified microfluidic chips and then the results were compared with a traditional counterpart technique of high-performance liquid chromatography (HPLC). The traditional process of separating cyclotides from plants is a costly and time-consuming procedure. The scientific novelty of this study is to accelerate the separation of cyclotides using modified microfluidic chips with low cost and high efficiency. The results revealed that a novel and simple microfluidic chip concept is an effective approach for separating the Vigno groups in the violet extract. We believe that the concept could potentially be utilized for further drug development process especially for anticancer studies by coupling bioassay chips as online procedures via reducing in time and cost compared with traditional offline methods.© TÜBİTAK.