基于非环式葫芦[n]烷基纳米海绵显著增强了体外和体内中替莫芬的光动力治疗效果。
Acyclic cucurbit[n]uril-based nanosponges significantly enhance the photodynamic therapeutic efficacy of temoporfin in vitro and in vivo.
发表日期：2023 Sep 18
作者：
Zizhen Zhao, Jingyu Yang, Yamin Liu, Shuyi Wang, Wei Zhou, Zhan-Ting Li, Dan-Wei Zhang, Da Ma
来源：
Cellular & Molecular Immunology
摘要：
基于超分子囊泡模板交联制备了无环状瓜烷[模]尿藤基纳米海绵。该纳米海绵能够封装临床上批准使用的光动力治疗 (PDT) 药物 temoporfin。当充满纳米海绵时，temoporfin 的 PDT 生物活性在 HeLa 癌细胞中增强了7.5倍，在 B16-F10 癌细胞中增强了20.8倍。通过共聚焦激光扫描显微镜和流式细胞仪确认，导致 PDT 效果显著改善的原因是增强了细胞摄取能力。动物实验显示，纳米海绵可大大增加 temoporfin 对肿瘤的抑制效果。体外和体内实验证明，纳米海绵无毒且生物相容性良好。
Acyclic cucurbit[n]uril-based nanosponges are prepared based on supramolecular vesicle-templated cross-linking. The nanosponges are capable of encapsulating the clinically approved photodynamic therapeutic (PDT) drug temoporfin. When loaded with nanosponges, the PDT bioactivity of temoporfin is enhanced 7.5-fold for HeLa cancer cells and 20.8 fold for B16-F10 cancer cells, respectively. The reason for the significant improvement in PDT efficacy is confirmed to be an enhanced cell uptake by confocal laser scanning microscopy and flow cytometry. Animal studies show that nanosponges could dramatically increase the tumor suppression effect of temoporfin. In vitro and in vivo experiments demonstrate that nanosponges are nontoxic and biocompatible.