近年来，癌症相关外泌体蛋白质分析方面的定量技术取得了重要进展。
Recent progress in quantitative technologies for the analysis of cancer-related exosome proteins.
发表日期：2023 Sep 18
作者：
Zhongwen Ma, Huiying Xu, Bang-Ce Ye
来源：
Cellular & Molecular Immunology
摘要：
外泌体是一种细胞外囊泡，对于细胞间通信和分子交换起着重要作用。因其丰富的生物信息和对癌细胞以及肿瘤微环境相互作用的贡献，癌症源性外泌体是癌症早期诊断和治疗监测的潜在理想生物标志物。然而，在传统检测技术中存在一些缺点，如低灵敏度和繁琐的步骤。此外，仅仅进行外泌体定量并不能准确区分癌症患者和健康人。因此，开发高效、准确、低成本的外泌体表面蛋白分析技术是必要且关键的。近年来，许多研究人员在这一领域提出了新的检测策略。本综述总结了定量分析癌症相关外泌体蛋白的最新技术进展，主要包括基于适配体、纳米材料和抗体的检测方法，并讨论了未来发展的路线图，旨在提供外泌体研究的创新视角。
Exosomes are a kind of extracellular vesicles, which play a significant role in intercellular communication and molecular exchange. Cancer-derived exosomes are potential and ideal biomarkers for the early diagnosis and treatment monitoring of cancers because of their abundant biological information and contribution to the interaction between cancer cells and the tumor microenvironment. However, there are a number of drawbacks, such as low sensitivity and tedious steps, in conventional detection techniques. Furthermore, exosome quantification is not enough to accurately distinguish cancer patients from healthy individuals. Therefore, developing efficient, accurate, and inexpensive exosome surface protein analysis techniques is necessary and critical. In recent years, a considerable number of researchers have presented novel detection strategies in this field. This review summarizes the recent progress in quantitative technologies for the analysis of cancer-related exosome proteins, mainly including the detection methods based on aptamers, nanomaterials, and antibodies, discusses a roadmap for future developments, and aims to offer an innovative perspective of exosome research.