将肽表位整合到自组装材料中是获取具有高抗原密度和改善效力的纳米疫苗的成功策略。本研究设计了含有MAGE-A3/PADRE表位的自组装肽，以生成功能性的治疗性纳米疫苗。为了获得更高的稳定性，通过工程肽的控制性自组装，配制了肽/聚合物混合纳米颗粒。纳米颗粒对人类红细胞和树突状细胞都表现出良好的生物相容性。将纳米颗粒与未成熟树突状细胞共培养，触发免疫效应，并最终激活CD8+细胞。分析了接种纳米颗粒后健康C57BL/6小鼠的特异性抗原和IgG抗体反应。体内结果表明，针对纳米疫苗的特异性免疫应答主要通过细胞途径介导。本研究表明，通过开发自组装肽-聚合物混合纳米结构，可以有效增强肽表位疫苗的免疫原性。©2023年，作者。

Integrating peptide epitopes in self-assembling materials is a successful strategy to obtain nanovaccines with high antigen density and improved efficacy. In this study, self-assembling peptides containing MAGE-A3/PADRE epitopes were designed to generate functional therapeutic nanovaccines. To achieve higher stability, peptide/polymer hybrid nanoparticles were formulated by controlled self-assembly of the engineered peptides. The nanoparticles showed good biocompatibility to both human red blood- and dendritic cells. Incubation of the nanoparticles with immature dendritic cells triggered immune effects that ultimately activated CD8 + cells. The antigen-specific and IgG antibody responses of healthy C57BL/6 mice vaccinated with the nanoparticles were analyzed. The in vivo results indicate a specific response to the nanovaccines, mainly mediated through a cellular pathway. This research indicates that the immunogenicity of peptide epitope vaccines can be effectively enhanced by developing self-assembled peptide-polymer hybrid nanostructures.© 2023. The Author(s).