细胞程序性死亡的最近发现形式——火凋毁（pyroptosis），在肿瘤治疗中起着关键作用。然而，火凋毁在肿瘤治疗中的机制仍然不清楚。先前的研究表明，火凋毁的发生通常伴随着反应性氧化物（ROS）的激增，其中ONOO-是其中一种ROS，并与众多疾病密切相关。因此，有必要研究ONOO-与火凋毁之间的潜在关联。本文利用一种高灵敏度和快速响应的近红外（NIR）探针Rd700-PN，用于探索ONOO-与火凋毁之间未揭示的关系。我们成功地利用Rd700-PN首次检测到细胞火凋毁过程中ONOO-的波动。此外，结果表明，Rd700-PN能够对体内化疗药物诱导的肿瘤火凋毁能力进行探测。值得注意的是，本研究为阐明ONOO-与火凋毁之间的相关性并筛选激活火凋毁的抗肿瘤药物提供了一种优秀的手段。版权所有©2023 Elsevier B.V.保留所有权利。

Pyroptosis, a recently discovered form of programmed cell death, plays a pivotal role in oncological treatment. Howbeit, the mechanisms underlying pyroptosis in tumor treatment remain unclear. Previous research has demonstrated that the occurrence of pyroptosis generally accompanies a surge of reactive oxygen species (ROS) generation, with ONOO- being one of these ROS and closely linked to numerous diseases. Therefore, it is imperative to investigate the potential association between ONOO- and pyroptosis. Herein, a highly sensitive and rapidly responsive near-infrared (NIR) probe, Rd700-PN, is fabricated for exploring unrevealed relationships between ONOO- and pyroptosis. We successfully harness Rd700-PN to detect ONOO- fluctuation during cellular pyroptosis for the first time. Furthermore, the results demonstrate that Rd700-PN can scout the chemotherapeutic drug's induction ability of tumor pyroptosis in vivo. Notably, this study provides an excellent means to shed light on the correlation between ONOO- and pyroptosis and to screen antitumor drugs activating pyroptosis.Copyright © 2023 Elsevier B.V. All rights reserved.