本研究集中在利用胸腺嘧啶、壳聚糖或氧化石墨烯纳米复合改性的玻碳电极（抗CEA/THi-CS-GO/GCE）上固定抗体，进行癌症监测的电化学免疫传感研究。利用超声处理制备了THi-CS-GO纳米复合材料，并通过SEM、FTIR和XRD分析其形态和晶体结构，结果显示嘧啶和壳聚糖分子与氧化石墨烯纳米片的顶部和底部均发生堆积相互作用。电化学实验表明，抗CEA、THi-CS-GO/GCE对CEA化合物具有出色的灵敏度和选择性。当发现峰电流的减小与CEA浓度的对数在从10-3至104 ng/mL的广泛范围内成正比时，检测限值确定为0.8 pg/mL。对人体血清样品中检测CEA的免疫传感器的应用能力进行的测试结果显示，酶联免疫吸附测定（ELISA）和差分脉动伏安法（DPV）的结果非常一致。所制备的免疫传感器在确定人体血清制备的真实样本和临床应用中，展现了足够的稳定性。版权所有 © 2023 Elsevier Inc.

The current study has focused on electrochemical immunosensing of carcinoembryonic antigen (CEA) employing an immobilized antibody on a thionine, chitosan, or graphene oxide nanocomposite modified glassy carbon electrode (anti-CEA/THi-CS-GO/GCE) as an indicator of cancer monitoring. THi-CS-GO nanocomposites were made using ultrasonication, and analyses of their morphology and crystal structure using SEM, FTIR, and XRD showed that thionine and chitosan molecules were intercalated with stacking interactions with both the top and bottom of GO nanosheets. Electrochemical experiments revealed anti-CEA, THi-CS-GO/GCE to have exceptional sensitivity and selectivity towards CEA compounds. The detection limit value was established to be 0.8 pg/mL when it was discovered that variations in the decrease peak current were directly proportional to the logarithm concentration of CEA over a wide range from 10-3 to 104 ng/mL. Results of testing the immunosensor's application capability for detecting CEA in a sample of human serum show that ELISA and DPV results are very congruent. The produced immunosensor demonstrated adequate immunosensor precision in determining CEA in prepared genuine samples of human serum and clinical applications.Copyright © 2023. Published by Elsevier Inc.