真菌是一种易于获取的天然产生彩色化合物的来源。这些化合物可能用作治疗癌细胞的放射增敏剂。本研究对曼戟星麴菌（Aspergillus nidulans）在马铃薯葡萄糖琼脂（PDA）培养基中的产色能力进行了检测。通过紫外光谱仪和气相色谱质谱（GC/MS）对色素进行了表征。研究了曼戟星麴菌色素提取物对人喉癌细胞株（HEp-2）的细胞毒性作用，在三个不同剂量（5、10和15 Gy）的γ射线下进行了研究。同时对正常Vero细胞研究了曼戟星麴菌色素的细胞毒性活性。通过流式细胞术研究了细胞凋亡情况。并对P53、Caspase 3和Bcl-2基因表达进行了定量分析。紫外光谱和GC/MS显示了曼戟星麴菌产生罗多平色素的能力。奈曼星麴菌色素处理的HEp-2细胞的IC50约为208 µg/mL。相比之下，当与色素+10 Gy γ射线处理时，IC50约为115 µg/mL。然而，对于正常细胞，则检测到较低的细胞毒性活性。色素（208 g/mL）处理导致了约50% ± 1.0的总细胞凋亡水平，并且相对于未处理的HEp-2细胞，P53的基因表达增加了2.3倍，Caspase 3的基因表达增加了1.84倍，而Bcl-2的基因表达减少了（相对于未处理的HEp-2细胞，Bcl-2的基因表达为0.63倍）。此外，处理色素（115 µg/mL）+10Gy的情况下，细胞凋亡水平约为47.41% ± 1.7，P53的基因表达增加了2.53倍，Caspase 3的基因表达增加了2.0倍，而Bcl-2的基因表达下调了（相对于未处理的HEp-2细胞，Bcl-2的基因表达为0.61倍）。本研究得出了结论：曼戟星麴菌色素的抗癌活性在10 Gy的电离辐射下得到了增强，并且对正常Vero细胞具有较低的细胞毒性。©2023年，BioMed Central，主编Springer Nature。

Fungi are a readily available source of naturally generated colored compounds. These compounds might be used as radiosensitizers for treating cancer cells.Aspergillus nidulans was examined for its color-producing ability in Potato dextrose agar (PDA) broth medium. The pigment was characterized by Ultraviolet (UV) spectrophotometer and Gas Chromatography Mass Spectrometry (GC/MS). Pigment extracts from A. nidulans were studied for their cytotoxic effects on the growth of human larynx carcinoma cell line (HEp-2) with or without exposure to γ-radiation at three different doses (5, 10, and 15 Gy). A. nidulans pigment cytotoxic activity was tested against normal Vero cells. Cell apoptosis was studied using flow cytometry. Gene expression of P53, Caspase 3 and Bcl-2 were quantified.Ultraviolet spectrum and GC/MS revealed the ability of Aspergillus nidulans to produce Rhodopin pigment. HEp-2 cells treated with A. nidulans pigment only give IC50 about 208 µg/ml. In contrast, when treated with the pigment +10 Gy γ-radiation, it give about 115 µg/ml. However, for normal cells, lower cytotoxic activity was detected. Treatment with pigment (208 g/mL) caused about 50% ± 1.0 total apoptosis level and gene expression of P53: 2.3 fold and Caspase 3: 1.84 fold in respect to untreated HEp-2), while Bcl-2 was decreased (Bcl-2: 0.63 fold in respect to untreated HEp-2). Furthermore, treated with pigment (115 µg/mL) + 10Gy caused about 47.41% ± 1.7 total apoptosis level and P53: 2.53 fold and Caspase 3: 2.0 fold in respect to untreated HEp-2, while Bcl-2 was downregulated (Bcl-2: 0.61 fold in respect to untreated HEp-2).This study concluded that the anti-cancer activity of Aspergillus nidulans pigment was enhanced by ionizing radiation at 10 Gy, as well as its low cytotoxic activity against normal Vero cells.© 2023. BioMed Central Ltd., part of Springer Nature.