再生医学主要依赖非同种移植，常常受到样本供应和伦理问题的限制。此外，患者需要服用免疫抑制药物以预防不良副作用。干细胞衍生的三维器官样体培养为移植和再生医学提供了新的选择。学者们将器官样体与组织工程技术相结合，以改善再现性、构成和通量的准确性，并进行基因修正，以实现更个性化的治疗。在这里，我们回顾了器官样体在再生医学中的可用应用以及目前该领域所面临的挑战。© 2023作者. 版权归属于牛津大学出版社. 保留所有权利。如需使用本文，请发送电子邮件至：journals.permissions@oup.com。

Regenerative medicine mainly relies on heterologous transplantation, often hindered by sample availability and ethical issues. Furthermore, patients are required to take immunosuppressive medications to prevent adverse side effects. Stem cell-derived 3D-organoid culture has provided new alternatives for transplantation and regenerative medicine. Scholars have combined organoids with tissue engineering technology to improve reproducibility, the accuracy of constitution and throughput, and genetic correction to achieve a more personalised therapy. Here, we review the available applications of organoids in regenerative medicine and the current challenges concerning this field.© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.