乳腺癌（BC）是全球癌症相关死亡的第二大原因。本研究旨在调查基于营养和运动的家庭式生活方式干预（基于MoviS临床试验（编号：NCT04818359））对二十名BC幸存者的肠道微生物组成的影响。通过16S rRNA基因测序、体测学、地中海饮食（MD）依从性和心脑血管代谢参数评估了干预前（Pre）和干预后（Post）的肠道菌群。观察到干预对MD依从性和心脑血管代谢参数有积极影响（干预前对比干预后）。干预后明显减少了变形菌门，这能通过调节能够减少炎症的微生物和改善寄主脂肪和糖代谢状况的其他微生物来重塑肠道微生物群落。报道了空腹血糖与肠球菌_vadinBB60（r = -0.62）、胰岛素与家庭态模式评估（HOMA）指数和丁酸茵科克氏菌属（分别为r = -0.72和-0.66）以及高密度脂蛋白胆固醇与大肠杆菌/志贺菌（r = -0.59）之间的显著负相关关系。此外，还发现了MD依从性与梭杆菌目_ND3007（r = 0.50）、粪链球菌属（r = 0.38）和丁酸爱蒙菌属（r = 0.39）之间的正相关关系。这些数据表明，采用健康的生活方式可能有助于改善影响肠道微生物调节与预防乳腺癌复发相关的多个生物参数。版权所有© 2023 Donati Zeppa, Natalucci, Agostini, Vallorani, Amatori, Sisti, Rocchi, Pazienza, Perri, Villani, Binda, Panebianco, Mencarelli, Ciuffreda, Ferri Marini, Annibalini, Lucertini, Bartolacci, Imperio, Virgili, Catalano, Piccoli, Stocchi, Emili and Barbieri。

Breast cancer (BC) is the second-leading cause of cancer-related death worldwide. This study aimed to investigate the effects of a 12-week home-based lifestyle intervention (based on nutrition and exercise) on gut microbial composition in twenty BC survivors of the MoviS clinical trial (protocol: NCT04818359).Gut microbiota analysis through 16S rRNA gene sequencing, anthropometrics, Mediterranean Diet (MD) adherence, and cardiometabolic parameters were evaluated before (Pre) and after (Post) the lifestyle intervention (LI).Beneficial effects of the LI were observed on MD adherence, and cardiometabolic parameters (pre vs post). A robust reduction of Proteobacteria was observed after LI, which is able to reshape the gut microbiota by modulating microorganisms capable of decreasing inflammation and others involved in improving the lipid and glycemic assets of the host. A significant negative correlation between fasting glucose and Clostridia_vadinBB60 (r = -0.62), insulin and homeostatic model assessment (HOMA) index and Butyricicoccus genera (r = -0.72 and -0.66, respectively), and HDL cholesterol and Escherichia/Shigella (r = -0.59) have been reported. Moreover, positive correlations were found between MD adherence and Lachnospiraceae_ND3007 (r = 0.50), Faecalibacterium (r = 0.38) and Butyricimonas (r = 0.39).These data suggest that adopting a healthy lifestyle, may contribute to ameliorate several biological parameters that could be involved in the prevention of cancer relapses through the modulation of gut microbiota.Copyright © 2023 Donati Zeppa, Natalucci, Agostini, Vallorani, Amatori, Sisti, Rocchi, Pazienza, Perri, Villani, Binda, Panebianco, Mencarelli, Ciuffreda, Ferri Marini, Annibalini, Lucertini, Bartolacci, Imperio, Virgili, Catalano, Piccoli, Stocchi, Emili and Barbieri.