设计快速简单的定量方法，用于早期检测HeLa细胞的廉价一次性电极，对于临床诊断和公共卫生非常理想。在本研究中，我们基于一次性铟锡氧化物（ITO）电极上纵向有序介孔二氧化硅膜（VMSFs）的门控分子传输，开发了一种无标记、敏感的用于检测HeLa细胞的电化学细胞传感器。由于HeLa癌细胞的膜上存在高亲和力的叶酸受体，叶酸（FA）功能化的VMSF能够通过与HeLa细胞对VMSF表面的特异性识别和粘附来调节电化学探针（Fe（CN）6 3-）的传输。此外，VMSF作为固体骨架，能够有效防止细胞与底层电极的直接接触，保持底层电极的活性，并促进Fe（CN）6 3-的扩散。一旦HeLa细胞特异性粘附到VMSF表面，Fe（CN）6 3-氧化还原探针在二氧化硅纳米通道中的传输受到阻碍，最终导致电化学响应减弱，并实现HeLa细胞定量测定，具有广泛的线性范围（101-105 细胞/mL）和低检测限（4 细胞/mL）。所提出的电化学细胞传感器在宫颈癌早期诊断方面具有极大的潜力。版权所有©2023曾、赵、杨、习、苏。

Designing fast and simple quantitative methods on cheap and disposable electrodes for the early detection of HeLa cells is highly desirable for clinical diagnostics and public health. In this work, we developed a label-free and sensitive electrochemical cytosensor for HeLa cell detection based on the gated molecular transport across vertically ordered mesoporous silica films (VMSFs) on the disposable indium tin oxide (ITO) electrode. As high affinity for a folate receptor existed on the membrane of HeLa cancer cells, folic acid (FA) functionalized VMSF could regulate the transport of electrochemical probe (Fe(CN)6 3-) by the specific recognition and adhesion of HeLa cells toward the VMSF surface. In addition, VMSF, served as a solid skeleton, is able to effectively prevent the direct contact of cells with the underlying electrode, remaining the underlying electrode activity and favoring the diffusion of Fe(CN)6 3-. Once specific adhesion of HeLa cells to the VMSF surface happens, Fe(CN)6 3- redox probe exhibits impeded transport in the silica nanochannels, ultimately resulting in the decreased electrochemical responses and realizing the quantitative determination of HeLa cells with a broad linear range (101-105 cells/mL) and a low limit of detection (4 cells/mL). The proposed electrochemical cytosensor shows a great potential application for the early diagnosis of cervical cancer.Copyright © 2023 Zeng, Zhao, Yang, Xi and Su.