基于广西姜黄根茎介导的银纳米颗粒的新绿色合成：一项抗肺癌活性的临床前试验研究。
Novel green synthesis of silver nanoparticles mediated by Curcumae kwangsiensis for anti-lung cancer activities: a preclinical trial study.
发表日期：2023
作者：
Zhihong Liu, Zhuohong Zhang, Xiaomei Du, Ying Liu, Abdullah Alarfaj, Abdurahman Hirad, Siddique Akber Ansari, Zhiguo Zhang
来源：
ANTIOXIDANTS & REDOX SIGNALING
摘要：
本研究描述了含有广西莪术叶水提液的银纳米颗粒的绿色合成、表征和细胞毒性、抗氧化剂和抗人肺癌活性。银纳米颗粒是通过将AgNO3溶液与莪术叶水提液混合来制备的。利用FE-SEM、FT-IR、TEM和UV-Vis对银纳米颗粒进行了表征。FE-SEM和TEM图像显示颗粒的平均直径为15-21 nm。MTT法用于常见的人类肺癌细胞系，即肺腺癌分化良好（HLC-1）、肺腺癌分化中等（LC-2/ad）和肺腺癌分化差（PC-14）细胞系，以研究银纳米颗粒的细胞毒性和抗人肺癌效应。它们对HLC-1、LC-2/ad和PC-14细胞系具有剂量依赖性的非常低的细胞活性和高抗人肺癌活性，对正常细胞系（HUVEC）没有任何细胞毒性。银纳米颗粒对HLC-1、LC-2/ad和PC-14细胞系的IC50值分别为249、187和152 μg/ml。在浓度为1000 μg/ml时，细胞毒性和抗人肺癌性能的最佳结果可见。银纳米颗粒在浓度为135 μg/ml时抑制了一半的DPPH分子。也许广西莪术叶水提液合成的银纳米颗粒具有显著的抗人肺癌潜力与其抗氧化活性有关。在临床试验研究中确认上述结果后，这种制剂可以用于治疗人类肺癌。版权：© 2021 Termedia & Banach.
The present work describes the green synthesis and characterization and cytotoxicity, antioxidant, and anti-human lung cancer activities of silver nanoparticles containing Curcumae kwangsiensis folium leaf aqueous extract.Ag nanoparticles were produced by mixing the AgNO3 solution with aqueous C. kwangsiensis folium leaf extract. Characterization of Ag nanoparticles was done by FE-SEM, FT-IR, TEM, and UV-Vis. FE-SEM and TEM images revealed an average diameter of 15-21 nm for the nanoparticles. MTT assay was used on common human lung cancer cell lines, i.e., lung well-differentiated bronchogenic adenocarcinoma (HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated adenocarcinoma (PC-14) cell lines, to survey the cytotoxicity and anti-human lung cancer effects of Ag nanoparticles.They had very low cell viability and high anti-human lung cancer activities dose-dependently against HLC-1, LC-2/ad, and PC-14 cell lines without any cytotoxicity towards the normal cell line (HUVEC). The IC50 values of Ag nanoparticles were 249, 187, and 152 μg/ml against HLC-1, LC-2/ad, and PC-14 cell lines, respectively. The best results of cytotoxicity and anti-human lung cancer properties were seen at the concentration of 1000 μg/ml. Ag nanoparticles inhibited half of the DPPH molecules in the concentration of 135 μg/ml. Maybe significant anti-human lung cancer potentials of Ag nanoparticles synthesized by C. kwangsiensis folium leaf aqueous extract against common human lung cancer cell lines are linked to their antioxidant activities.After confirming the above results in the clinical trial research, this formulation can be administered to treat human lung cancers in humans.Copyright: © 2021 Termedia & Banach.