艾尔皮尼唑是一种传统中药的生物活性成分。这种化合物是青蒿剑叶花穗Alpinia katsumadai Hayata种子的主要成分之一，属于黄酮类物质，具有重要的抗炎、抗菌和其他显著疗效的药理活性，并具有重要的效能和低系统毒性。在我们的研究中，对异甘草苷酮对HMG-CoA还原酶的抑制作用结果显示，IC50值为21.86 ±1.44 μg/ml。作为补充研究，进行了分子对接研究，以提供关于在尿素酶存在下艾尔皮尼唑的生物活性的附加数据。对接计算显示，艾尔皮尼唑的对接评分为-5.097（kcal/mol），与该酶具有可接受的结合亲和力，由于该化合物所造成的各种疏水接触和氢键作用，艾尔皮尼唑可以被视为尿素酶的足够抑制剂。 在细胞和分子方面的研究中，用MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴化物）法评估了艾尔皮尼唑处理的细胞，针对正常（人脐静脉内皮细胞（HUVECs））和胃癌细胞系（SNU-1，Hs 746T和KATO III）进行了48小时的细胞毒性和抗人胃癌特性的评估。艾尔皮尼唑对SNU-1，Hs 746T和KATO III细胞系的IC50值分别为426、586和424μg/ml。恶性胃癌细胞系的存活能力在艾尔皮尼唑存在下呈剂量依赖性下降。 这种研究物质对抗人胃癌的作用似乎是由其抗氧化作用引起的。版权所有：© 2021 Termedia & Banach。

Alpinetin is the bioactive component of a traditional Chinese medicine. This compound, one of the main constituents of the seeds of Alpinia katsumadai Hayata, is a member of the flavonoids, with anti-inflammatory, antibacterial, and other significant therapeutic activities of important potency and low systemic toxicity.In our study, the inhibitory effect of isoliquiritigenin on HMG-CoA reductase showed a lower value of IC50 = 21.86 ±1.44 μg/ml. A molecular docking study was performed as a complementary study to provide additional data about the biological activities of alpinetin in the presence of urease. The docking calculations revealed that alpinetin with a docking score of -5.097 (kcal/mol) has an acceptable binding affinity to the enzyme, and because of various hydrophobic contacts and hydrogen bonds created by this chemical compound, alpinetin could be considered as an adequate inhibitor of urease.In the cellular and molecular part of the study, the cells treated with alpinetin were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay for 48 h as regards the cytotoxicity and anti-human gastric carcinoma properties towards normal (human umbilical vein endothelial cells (HUVECs)) and gastric carcinoma cell lines, i.e. SNU-1, Hs 746T, and KATO III. The IC50 values of alpinetin were 426, 586, and 424 μg/ml against SNU-1, Hs 746T, and KATO III cell lines, respectively. The viability of the malignant gastric cell line decreased dose-dependently in the presence of alpinetin.It seems that the anti-human gastric carcinoma effect of the investigated molecule is due to its antioxidant effects.Copyright: © 2021 Termedia & Banach.