一直以来，人们一直认为一种植物具有很长的潜力，这种植物是大头蕨（Equisetum arvense L）。现在推荐将大头蕨外用于治疗创伤，并内服于缓解尿路和前列腺疾病。本研究中，我们研究了大头蕨在体外条件下的抗氧化、细胞毒性和抗人肺癌特性。通过评估总酚含量、总黄酮含量、自由基清除活性和亚铁离子螯合来评估其抗氧化活性。选择3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴化物（MTT）试验来研究植物提取物的抗癌活性。该植物提取物以IC50为12.3±0.7 µg/ml清除了2,2-二苯基-1-哌啶基自由基（DPPH），比阳性对照效果更好。该植物也富含酚类化合物，总酚含量为396.2±3.2 mg GAE/g。在MTT试验中，我们使用人结肠癌（HCT-8 [HRT-18]，Ramos.2G6.4C10，HT-29和HCT 116）和正常细胞系（HUVEC）研究了大头蕨对人结肠癌的细胞毒性和抗癌潜力。大头蕨对人结肠癌细胞系的细胞存活率很低，而对正常（HUVEC）细胞系没有细胞毒性。在以上细胞系中，大头蕨对HT 29细胞系表现出最佳的抗人结肠癌特性。版权所有：© 2021 Termedia & Banach.

One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-human lung cancer properties of Equisetum arvense were investigated in in vitro conditions.Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were assessed to evaluate the antioxidant activity. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was chosen to investigate anticancer activity of the plant extract.The plant extract scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical with an IC50 of 12.3 ±0.7 µg/ml better than positive controls. The plant was also rich in phenolic compounds with an amount of 396.2 ±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of Equisetum arvense L against human colorectal cancer.The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity towards the normal (HUVEC) cell line. The best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines were observed in the case of the HT 29 cell line.Copyright: © 2021 Termedia & Banach.