非小细胞肺癌(NSCLC)的个体化治疗已经取得了快速发展，阐明引发该疾病的基因变化对于适当的治疗选择至关重要。慢拉式和吸引式内支气管超声引导经纤维支气管镜穿刺活检(EBUS-TBNA)是采集适于下一代测序(NGS)检测NSCLC驱动基因突变和易位的经验性方法。本研究旨在确定这两种方法中，哪一种更适合从NSCLC患者获得高质量的样本。 2019年7月至2022年9月，使用慢拉式或吸引(负压20 mL)方法经EBUS-TBNA诊断出NSCLC的71名患者被纳入研究。共收集了71名患者的203个组织样本，经福尔马林固定，包埋于石蜡中，并制作成玻璃切片。比较了两组之间的组织样本核心存在情况、血液污染程度和肿瘤细胞数量，并比较了NGS(使用Oncomine Dx Target Test Multi-CDx)的成功率。 慢拉式方法与吸引式方法相比，组织样本核心的产量更高，血液污染程度较低，肿瘤细胞数量较多。慢拉式组的NGS成功率(95%)也显著高于吸引式组(68%)。 总的来说，这些发现表明，慢拉式方法是一种更优越的EBUS-TBNA技术，可获得用于NGS的高质量组织样本。慢拉式方法可能有助于鉴定驱动基因突变和易位，并促进NSCLC的个体化治疗。 © 2023 The Authors. 癌症药物由John Wiley & Sons Ltd发表。

Personalized treatment for non-small cell lung cancer (NSCLC) has advanced rapidly, and elucidating the genetic changes that trigger this disease is crucial for appropriate treatment selection. Both slow-pull and aspiration methods of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) are accepted methods for collecting samples suitable for next-generation sequencing (NGS) to examine driver gene mutations and translocations in NSCLC. Here, we aimed to determine which of these two methods is superior for obtaining higher-quality samples from patients with NSCLC.Seventy-one patients diagnosed with NSCLC via EBUS-TBNA using the slow-pull or aspiration (20-mL negative pressure) methods between July 2019 and September 2022 were included. A total of 203 tissue samples from the 71 patients were fixed in formalin, embedded in paraffin, and mounted on slides. The presence of tissue cores, degree of blood contamination, and number of tumor cells were compared between the groups. The success rate of NGS, using Oncomine Dx Target Test Multi-CDx, was also compared between the groups.The slow-pull method was associated with a higher yield of tissue cores, lower degree of blood contamination, and higher number of tumor cells than the aspiration method. The success rate of the NGS was also significantly higher for the slow-pull group (95%) than for the aspiration group (68%).Overall, these findings suggest that the slow-pull method is a superior technique for EBUS-TBNA to obtain high-quality tissue samples for NGS. The slow-pull method may contribute to the identification of driver gene mutations and translocations and facilitate personalized treatment of NSCLC.© 2023 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.