Cxs在维持晶状体透明度方面起着关键作用。在这里，我们提出了一种使用高滴度反转录病毒复制激活鸟肉瘤-白血病病毒长末端重复序列与剪切子（A）感染改变初级鸡晶状体纤维细胞Cx半通道（HC）功能的协议。我们描述了孵化卵、分离晶状体、培养细胞、制备试剂和感染细胞的步骤。然后我们详细介绍了细胞处理以及检测凋亡和死亡。该方案可以评估蛋白激酶A、HC活性和增加谷胱甘肽转运以保护晶状体纤维细胞免受氧化应激的影响。关于使用和执行该协议的完整细节，请参考Liu等人（1）、Riquelme等人（2）、Shi等人（3）、Jiang（4）和Rath等人（5）。版权所有 © 2023 The Authors. Elsevier Inc.发表。保留所有权利。

Connexins (Cxs) play a crucial role in maintaining lens transparency. Here, we present a protocol for altering Cx hemichannel (HC) function in primary chicken lens fiber cells using high-titer retroviral replication competent avian sarcoma-leukosis virus long terminal repeat with splice acceptor (A) infection. We describe steps for incubating eggs, isolating lenses, culturing cells, preparing reagents, and infecting cells. We then detail cell treatment and detection of apoptosis and death. This protocol can assess protein kinase A, HC activity, and increased glutathione transport for protecting lens fiber cells against oxidative stress. For complete details on the use and execution of this protocol, please refer to Liu et al.,1 Riquelme et al.,2 Shi et al.,3 Jiang,4 and Rath et al.5.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.