Beclin1（Becn1）是一种参与自噬调节、膜转运和肿瘤抑制的多功能蛋白。在本研究中，我们通过使用胰腺转录因子1a（Ptf1a）-Cre小鼠（Becn1f/f; Ptf1aCre/+）在胰腺中特异性删除Becn1来研究其在胰腺发育中的作用。令人惊讶的是，胰腺中Becn1的丧失导致严重的胰腺发育缺陷，进而导致外分泌和内分泌胰腺功能不足。约一半的Becn1f/f; Ptf1aCre/+小鼠在出生后立即死亡。然而，十二指肠和神经组织的发育几乎正常，表明胰腺功能不足是导致死亡的原因。这些发现揭示了Becn1在胰腺形态发生、分化和生长中的新角色，并暗示失去该因子会导致出生时胰腺缺失。版权所有 © 2023。由Elsevier Inc.出版。

Beclin1 (Becn1) is a multifunctional protein involved in autophagy regulation, membrane trafficking, and tumor suppression. In this study, we examined the roles of Becn1 in the pancreas development by generating mice with conditional deletion of Becn1 in the pancreas using pancreatic transcriptional factor 1a (Ptf1a)-Cre mice (Becn1f/f; Ptf1aCre/+). Surprisingly, loss of Becn1 in the pancreas resulted in severe pancreatic developmental defects, leading to insufficient exocrine and endocrine pancreatic function. Approximately half of Becn1f/f; Ptf1aCre/+ mice died immediately after birth. However, duodenum and neural tissue development were almost normal, indicating that pancreatic insufficiency was the cause of death. These findings demonstrated a novel role for Becn1 in pancreas morphogenesis, differentiation, and growth, and suggested that loss of this factor leaded to pancreatic agenesis at birth.Copyright © 2023. Published by Elsevier Inc.