近期研究显示循环RNA的重要调控作用，但跨不同物种全面了解循环RNA的景观仍未被探索。当前的循环RNA数据库通常受限于物种或基于过时的数据集。为了应对这一挑战，我们开发了circAtlas 3.0数据库，其中包含2674个循环RNA测序数据集，经过筛选，用于描述包括10个脊椎动物物种在内的33种组织中的循环RNA景观。值得注意的是，circAtlas 3.0相较于其前身circAtlas 2.0取得了实质性进展，目录化的循环RNA数量从1,007,087增加到3,179,560，其中2,527,528被重建成全长异构体。circAtlas 3.0还引入了几个重要的改进，包括：（i）整合Illumina和Nanopore测序数据集以检测更长的循环RNA；（ii）采用标准化的命名方案，提供循环RNA的宿主基因和全长环状外显子的信息；（iii）包括临床癌症样本，以探索循环RNA在癌症背景下的生物学功能；（iv）链接到其他有用资源，以便用户友好地分析目标循环RNA。升级版的circAtlas 3.0为研究脊椎动物循环RNA的进化和生物学意义提供了重要的平台，并可免费在http://circatlas.biols.ac.cn和https://ngdc.cncb.ac.cn/circatlas 上获取。© 作者2023。牛津大学出版社代表核酸研究（Nucleic Acids Research）发表。

Recent studies have demonstrated the important regulatory role of circRNAs, but an in-depth understanding of the comprehensive landscape of circRNAs across various species still remains unexplored. The current circRNA databases are often species-restricted or based on outdated datasets. To address this challenge, we have developed the circAtlas 3.0 database, which contains a rich collection of 2674 circRNA sequencing datasets, curated to delineate the landscape of circRNAs within 33 distinct tissues spanning 10 vertebrate species. Notably, circAtlas 3.0 represents a substantial advancement over its precursor, circAtlas 2.0, with the number of cataloged circRNAs escalating from 1 007 087 to 3 179 560, with 2 527 528 of them being reconstructed into full-length isoforms. circAtlas 3.0 also introduces several notable enhancements, including: (i) integration of both Illumina and Nanopore sequencing datasets to detect circRNAs of extended lengths; (ii) employment of a standardized nomenclature scheme for circRNAs, providing information of the host gene and full-length circular exons; (iii) inclusion of clinical cancer samples to explore the biological function of circRNAs within the context of cancer and (iv) links to other useful resources to enable user-friendly analysis of target circRNAs. The updated circAtlas 3.0 provides an important platform for exploring the evolution and biological implications of vertebrate circRNAs, and is freely available at http://circatlas.biols.ac.cn and https://ngdc.cncb.ac.cn/circatlas.© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.