从临床样本中分离出产生铁载体的新种Pantoea ferrattrahens，以及从池塘底部的土壤中分离出的新种Pantoea ferramans。
Siderophore-producing Pantoea ferrattrahens sp. nov. isolated from a clinical specimen and Pantoea ferramans sp. nov. isolated from soil at the bottom of a pond.
发表日期：2023 Sep 22
作者：
Ryo Kutsuna, Tohru Miyoshi-Akiyama, Yuki Muramatsu, Moriyuki Hamada, Junko Tomida, Ken Kikuchi, Yoshiaki Kawamura
来源：
GENES & DEVELOPMENT
摘要：
从胰腺癌脓毒症患者（PAGU 2156T菌株）和塘底土壤（PAGU 2198T菌株）中分离出两种革兰阴性厌氧培养菌。这两种菌株在铬铬天蓝S琼脂培养基平板上形成菌落周围的光晕，表明它们产生铁载体。基于16S rRNA基因，两种分离菌株被归类为假单胞菌属。通过使用多态分类法，将这两种分离菌株与已有物种区分开来。通过使用gyrB、rpoB、atpD和infB四个基因进行的系统发育分析显示，PAGU 2156T株菌与CBasp.P. Cypripedii LMG 2657T（89.9%）或CBasp.P. Septica LMG 5345T（95.7%）密切相关。与此同时， PAGU 2198T株菌与CBasp.P. Rodasii DSM 26611T（93.6%）和CBasp.P. Rwandensis DSM 105076T（93.3%）形成了单一支系。通过对草稿基因组组装获得的平均核苷酸同一性值显示，PAGU 2156T株菌与密切相关物种之间的同一性值≤90.2%，而PAGU 2198T株菌与密切相关物种之间的同一性值≤81.5%。基于各种表型、生化特性和与相关物种相比的整细胞脂肪酸组分，得出结论，应将每个菌株归类为假单胞菌属的新物种。在本文中，建议将PAGU 2156T株（=NBRC 115930T = CCUG 76757T）命名为 Pantoea ferrattrahens sp. nov.，将PAGU 2198T株（=NBRC 114265T = CCUG 75151T）命名为 Pantoea ferramans sp. nov.。 ©2023 The Societies and John Wiley & Sons Australia, Ltd.
Two Gram-negative facultative anaerobes were isolated from a sepsis patient with pancreatic cancer (strain PAGU 2156T ) and soil at the bottom of a pond (strain PAGU 2198T ), respectively. These two strains formed haloes around the colonies on chrome azurol S agar plates, indicating the production of siderophores. Two isolates assigned to the genus Pantoea based on the 16S rRNA gene were differentiated from established species by using polymorphic taxonomies. Phylogenetic analysis using four housekeeping genes (gyrB, rpoB, atpD, and infB) showed that strain PAGU 2156T is closely related to Pantoea cypripedii LMG 2657T (89.9%) or Pantoea septica LMG 5345T (95.7%). Meanwhile, strain PAGU 2198T formed a single clade with Pantoea rodasii DSM 26611T (93.6%) and Pantoea rwandensis DSM 105076T (93.3%). The average nucleotide identity values obtained from the draft genome assembly showed ≤90.2% between strain PAGU 2156T and closely related species and ≤81.5% between strain PAGU 2198T and closely related species. Based on various phenotypes, biochemical properties, and whole-cell fatty acid composition compared with related species, it was concluded that each strain should be classified as a new species of the genus Pantoea. In this manuscript, Pantoea ferrattrahens sp. nov. and Pantoea ferramans sp. nov. with strain PAGU 2156T (=NBRC 115930T = CCUG 76757T ) and strain PAGU 2198T (=NBRC 114265T = CCUG 75151T ) are proposed as each type strain.© 2023 The Societies and John Wiley & Sons Australia, Ltd.