脑部内的微量元素对于正常的神经功能至关重要，但在胶质母细胞瘤中它们的不平衡鲜为人知。本研究纳入了14名患有胶质母细胞瘤的患者，手术过程中采集了肿瘤和肿瘤周围脑组织样本。采用经过良好评估的ICP-MS方法测定了Mg，Ca，Cr，Mn，Fe，Co，Cu，Zn，Se，As，Cd，Tl和Pb的浓度。进一步使用Ivy GAP的解剖草图对Cu和Cd的结合蛋白组进行了分析。组织学评估分别基于鲁比那酸染色和免疫组织化学。得到了13种微量元素的浓度，其中Ca，Mn，Fe，Zn和Cu的浓度最高，从几个ug/g到几十个ug/g不等。相关分析表明存在两个内部相关的簇：必需金属（Cu-Ca-Zn-Mg）和重金属（Pb-As-Cd-Tl-Co-Cr-Mn）。与肿瘤样本相比，周围肿瘤区域观察到明显较高的Cu和Cd水平。通过解剖角度对Cu和Cd结合蛋白进行进一步分析表明，与中心肿瘤区域相比，DBH和NOS1在前沿区明显增加。与上述发现一致，Cu和DBH的组织学评估进一步证实了与肿瘤核心相比，在周围肿瘤区域中表达更多的铜和DBH。胶质母细胞瘤的肿瘤和周围脑区的微量元素存在差异，可能与肿瘤血管生成相关。 © 2023. 作者，独家许可给生物无机化学学会（SBIC）。

Trace elements within the brain are important for proper neurological function, but their imbalance has been rarely investigated in glioblastoma. This study enrolled a total of 14 patients with glioblastoma, and the tumor and peritumoral brain tissues were collected while undergoing surgery. The concentrations of Mg, Ca, Cr, Mn, Fe, Co, Cu, Zn, Se, As, Cd, Tl and Pb were determined using a well-evaluated ICP-MS method. The Cu- and Cd-binding proteomes were further analyzed using the anatomic transcriptional atlas from Ivy GAP. Histological evaluation was based on rubeanic acid staining and immunohistochemistry, respectively. The 13 trace element concentrations were obtained, and the highest were Ca, Mn, Fe, Zn and Cu, ranging from a few to dozens of ug/g. Correlation analysis suggested the existence of two intra-correlated clusters: essential metals (Cu-Ca-Zn-Mg) and heavy metals (Pb-As-Cd-Tl-Co-Cr-Mn). Compared to the tumor samples, significantly higher levels of Cu and Cd were observed in the peritumoral region. Further analysis of the Cu- and Cd-binding proteins from the anatomic view suggested that DBH and NOS1 were obviously increased in the leading edge than the central tumor region. Consistent with the above findings, histological evaluation of Cu and DBH further confirmed more copper and DBH expressions in the peritumoral area compared to the tumor core. Trace elements differ in tumor and peritumoral brain zone in glioblastoma, which may associate with tumor angiogenesis.© 2023. The Author(s), under exclusive licence to Society for Biological Inorganic Chemistry (SBIC).