癌基因MYCN与神经母细胞瘤（NB）的恶性进展和不良预后密切相关。最近，长链非编码RNA（lncRNA）在各种癌症中被认为是关键调节因子。然而，lncRNA是否对NB中MYCN的过表达有贡献还不清楚。我们应用微阵列分析来分析MYCN扩增和MYCN非扩增的NB细胞系之间的差异表达lncRNA。利用生物信息学分析方法鉴定了MYCN基因座附近的lncRNA。使用qRT-PCR检测了NB细胞系和组织中lncRNA AC142119.1的表达水平。进行了增强功能和失去功能实验，以研究AC142119.1在NB中的生物学效应。进行了荧光原位杂交、RNA pull-down、RNA免疫沉淀、质谱、RNA电泳迁移、染色质免疫沉淀和染色质RNA分离等实验，以验证AC142119.1与WDR5蛋白及MYCN启动子之间的相互作用。AC142119.1在MYCN扩增、晚期INSS分期和高风险的NB组织中显著升高，并与NB患者的不良生存率相关。此外，AC142119.1的过表达在体外和体内均加强了NB细胞的增殖。此外，AC142119.1能够特异性地招募WDR5蛋白与MYCN启动子相互作用，进一步促进MYCN的转录并加速NB的进展。我们鉴定了一个新的lncRNA AC142119.1，通过与WDR5蛋白和MYCN启动子相互作用，表触发MYCN的转录，从而促进NB的进展。这表明AC142119.1可能是NB的潜在诊断生物标记和治疗靶点。©2023. BioMed Central有限公司，Springer Nature的一部分。

Oncogene MYCN is closely related with malignant progression and poor prognosis of neuroblastoma (NB). Recently, long non-coding RNAs (lncRNAs) have been recognized as crucial regulators in various cancers. However, whether lncRNAs contribute to the overexpression of MYCN in NB is unclear.Microarray analysis were applied to analyze the differentially expressed lncRNAs between MYCN-amplified and MYCN-non-amplified NB cell lines. Bioinformatic analyses were utilized to identify lncRNAs nearby MYCN locus. qRT-PCR was used to detect the expression level of lncRNA AC142119.1 in NB cell lines and tissues. Gain- and loss-of-function assays were conducted to investigate the biological effect of AC142119.1 in NB. Fluorescence in situ hybridization, RNA pull-down, RNA immunoprecipitation, mass spectrometry, RNA electrophoretic mobility shift, chromatin immunoprecipitation and chromatin isolation by RNA purification assays were performed to validate the interaction between AC142119.1 and WDR5 protein as well as MYCN promoter.AC142119.1 was significantly elevated in NB tissues with MYCN amplification, advanced INSS stage and high risk, and associated with poor survival of NB patients. Moreover, enforced expression of AC142119.1 reinforced the proliferation of NB cells in vitro and in vivo. Additionally, AC142119.1 specifically recruited WDR5 protein to interact with MYCN promoter, further initiating the transcription of MYCN and accelerating NB progression.We identified a novel lncRNA AC142119.1, which promoted the progression of NB through epigenetically initiating the transcription of MYCN via interacting with both WDR5 protein and the promoter of MYCN, indicating that AC142119.1 might be a potential diagnostic biomarker and therapeutic target for NB.© 2023. BioMed Central Ltd., part of Springer Nature.