免疫疗法在临床前和临床研究中取得了一定的成功，但免疫抑制性肿瘤微环境（TME）导致该疗法的低响应率。本文描述了一种来自P. aeruginosa（CPW / SR）的镀儿茶酸（CRT）-26肿瘤细胞膜包被的细菌全肽聚糖（WPG），具有高STING激动剂载荷率。在该结构中，来自P. aeruginosa（P.WPG）的WPG被用作具有免疫佐剂作用的载体，同时通过输送STING激动剂SR-717来协同促进树突状细胞（DCs）的成熟，而CRT镀正肿瘤细胞膜通过表面的CRT被DCs鉴定和内化。此外，该结构能够逆转体内的免疫抑制性TME，并通过创建个性化肿瘤疫苗与放疗协同作用，从而实现更为有效的抗肿瘤疗效。总之，本文构建的CPW / SR为实现高效癌症免疫治疗和联合治疗提供了一种新方法。版权所有 © 2023. Elsevier B.V. 发表。

Immunotherapy has achieved some success in preclinical and clinical studies, but the immunosuppressive tumor microenvironment (TME) leads to a low response rate of this therapy. In this paper, we describe a calreticulin (CRT) valgus CT-26 tumor cell membranes-coated bacterial whole peptidoglycan (WPG) from P. aeruginosa (CPW/SR) with a high rate of the STING agonist loading. In the construct, WPG from P. aeruginosa (P.WPG) was used as a carrier with the immunoadjuvant function while synergistically promoting the maturation of dendritic cells (DCs) through the delivery of the STING agonist SR-717. CRT valgus tumor cell membranes were identified and internalized by DCs via CRT on the surface. In addition, this construct was able to reverse the immunosuppressive TME in vivo and achieve synergies with radiotherapy by creating a personalized tumor vaccine, therefore achieving more resultful antitumor efficacy. In conclusion, CPW/SR constructed in this paper provides a new approach for achieving efficient cancer immunotherapy and combination therapy.Copyright © 2023. Published by Elsevier B.V.