具有不断上升的全球发病率的肝癌是一个实现有效治疗结果的重大挑战。作为食物来源的营养物质，海参肽（SCP）显示出了良好的抗癌效果。因此，我们探索了纳米传递系统来封装SCP，以增强其在胃肠道内的稳定性并改善在肿瘤微环境中的吸收。本研究旨在利用一锅曼尼希缩合法通过分子组装制备可控尺寸的多功能纳米颗粒，该方法使用SCP、原花青素（PCs）和香草醛进行分子组装。这些食品级纳米颗粒具有水溶性，并呈现出球形结构，尺寸从441到1360 nm不等，取决于反应物的浓度。体外细胞实验表明，修饰了PCs的SCP纳米颗粒有效降低了H2O2和丙烯酰胺产生的活性氧自由基，同时保持了正常水平的线粒体膜电位。此外，对肿瘤小鼠进行的体内营养干预研究显示，接受SCP纳米颗粒处理的小鼠的存活率为40％，明显高于对照组和SCP处理组的0％和20％的存活率。这些发现表明，具有抗氧化特性和可控大小的SCP纳米颗粒在癌症治疗领域具有精确营养的潜力。版权© 2023 Elsevier B.V. 出版。

Liver cancer, a malignancy with a rising global incidence, poses a significant challenge in achieving effective treatment outcomes. As food-derived nutrient, sea cucumber peptide (SCP) has shown promising anticancer effects. Therefore, we explored the nanodelivery systems to encapsulate SCP to enhance its stability in the gastrointestinal tract and improve absorption within the tumor microenvironment. This study aimed to develop size-controllable multifunctional nanoparticles using SCP, procyanidins (PCs), and vanillin through molecular assembly via a one-pot Mannich condensation approach. These food-grade nanoparticles demonstrated water solubility and exhibited a spherical structure with sizes ranging from 441 to 1360 nm, depending on the concentration of the reactants. In vitro cell experiments demonstrated that SCP nanoparticles modified with PCs effectively reduced the generation of reactive oxygen species from H2O2 and acrylamide while maintaining normal levels of mitochondrial membrane potential. Furthermore, in vivo nutrition intervention studies conducted on tumor-bearing mice revealed that mice treated with SCP nanoparticles exhibited a survival rate of 40 %, which was significantly higher than the 0 % and 20 % survival rates observed in the control and SCP-treated groups, respectively. These findings suggest that SCP nanoparticles, possessing antioxidative properties and controllable sizes, hold potential for precision nutrition in the field of cancer treatment.Copyright © 2023. Published by Elsevier B.V.