自采样的人乳头瘤病毒（HPV）检测是一种潜在的宫颈癌筛查选择，然而，柬埔寨在该领域的研究仍然很少。我们评估了使用careHPV进行自采样HPV检测的可行性、准确性和可接受性。我们进行了一项横断面研究，包括在首都的两家国家医院接受检查的年龄在20至49岁之间的妇女。妇女们进行了自采样和临床人员采样，并被要求填写一份可接受性问卷。通过careHPV对配对样本进行高危HPV检测，并通过聚合酶链反应（PCR）进行基因型分析。共有375名妇女符合纳入条件。根据PCR检测，78.9%的妇女在自采样和临床人员采样中都呈阴性，9.9%的妇女完全匹配了HPV类型，6.1%的妇女在临床人员采样中检测到的所有HPV类型也在自采样中检测到。5.1%的妇女在自采样中错过了在临床人员采样中检测到的一个或多个HPV类型。使用careHPV时，两种采样方法的总一致性为95.7%（95%可信区间[CI]=95.8-95.6），一致性良好（κ=0.66，95% CI=0.56-0.76）。近90%的妇女更喜欢临床人员采样，原因是感觉更舒适、简便和快捷。在柬埔寨，使用careHPV进行自采样HPV检测可能是一种宫颈癌筛查的选择，但需要定期质量控制处理程序。此外，需要向妇女提供关于自采样HPV检测准确性以及积极结果时后续随访重要性的健康教育。© 2023. 亚洲妇产科肿瘤学会，韩国妇产科肿瘤学会和日本妇产科肿瘤学会。

Self-sampled human papillomavirus (HPV) testing is a potential option for cervical cancer screening, but research is scarce in Cambodia. We evaluated the feasibility, accuracy, and acceptability of self-sampled HPV testing using careHPV.A cross-sectional study including women aged 20-49 years attending 2 national hospitals in the capital city was conducted. Women underwent both self-sampling and clinician-sampling of specimens, and were then asked to complete an acceptability questionnaire. The paired samples were analyzed for high-risk HPV by careHPV and genotyped by polymerase chain reaction (PCR).A total of 375 women were eligible for inclusion. Based on PCR, 78.9% were negative for HPV in both self and clinician-samples, 9.9% had a complete HPV type match, and 6.1% had all HPV types in clinician-samples also detected in self-samples. In 5.1%, one or more HPV types identified in the clinician-samples were missed in self-samples. When using careHPV, the overall agreement between the 2 sampling methods was 95.7% (95% confidence interval [CI]=95.8-95.6) with good concordance (κ=0.66, 95% CI=0.56-0.76). Nearly 90% of the women preferred clinician-sampling over self-sampling, citing greater comfort, ease, and speed.Self-sampled HPV testing using careHPV could be an option for cervical cancer screening in Cambodia; however, it requires periodic quality control of handling procedures. In addition, women's health education regarding the accuracy of self-sampled HPV testing and the importance of follow-up in cases of positive results is needed.© 2023. Asian Society of Gynecologic Oncology, Korean Society of Gynecologic Oncology, and Japan Society of Gynecologic Oncology.