纳米结构在新型医疗和药物输送应用方面具有巨大潜力。本文研究了一种利用 Prosopis fracta 提取物制备纯氧化镍 (NiO) 和 5% 钴掺杂 NiO (Co╫NiO) 纳米颗粒 (NPs) 的绿色方法。通过 PXRD、Raman、UV-Vis、FESEM 和 EDX 分析证明了 Co╫NiO NPs 的产物。 XRD、EDX 和紫外-可见光谱结果显示 NiO NP 中钴掺杂良好。观察到Co=NiO NPs 的粒径约为80 nm。 Co╫NiO NPs对乳腺癌细胞（MCF-7）的细胞毒性的MTT测试结果证实，与NiO NPs相比，掺杂的NiO-NPs对癌细胞的影响更强。因此，表明NiO NPs的掺杂过程导致其对MCF-7细胞的抑制作用增强。研究亮点：采用环保合成方法制备钴掺杂 NiO 纳米粒子，并研究其对 MCF-7 细胞的细胞毒性。© 2023 Wiley periodicals LLC。

The nanostructures have the great potential for novel medical and drug delivery applications. In present paper a green approach for the preparation of pure nickel oxide (NiO) and 5% cobalt-doped NiO (Co╫NiO) nanoparticles (NPs) by using Prosopis fracta extract have been study. The product of Co╫NiO NPs was proved through the PXRD, Raman, UV-Vis, FESEM, and EDX analyses. The results of XRD, EDX, and UV-Visible spectra displayed well doped cobalt in NiO NP. The particle sizes of Co╫NiO NPs were observed to be about 80 nm. The MTT test results for the cytotoxicity of Co╫NiO NPs on breast cancer cells (MCF-7) affirmed the stronger impact of doped NiO-NPs on cancer cells compared to NiO NPs. Thus, it is indicated that the doping process on NiO NPs caused an increase in its inhibitory effect against MCF-7 cells. RESEARCH HIGHLIGHTS: Cobalt-doped NiO nanoparticles were prepared using ecofriendly synthesis method and their cytotoxicity studied against MCF-7 cells.© 2023 Wiley Periodicals LLC.