禽肉瘤/白血病病毒 (RCAS) 介导的 IFITM3 过度表达可保护雏鸡免受高致病性禽流感病毒 H5N1 亚型的感染。
Avian sarcoma/leukosis virus (RCAS)-mediated over-expression of IFITM3 protects chicks from highly pathogenic avian influenza virus subtype H5N1.
发表日期：2023 Sep 28
作者：
Mohammed A Rohaim, Emma Louise Gardiner, Rania F El Naggar, Mohammed A Abdelsabour, Yahia M Madbouly, Mustafa O Atasoy, Kawkab A Ahmed, Munir M El-Safty, Muhammad Munir
来源：
MICROBES AND INFECTION
摘要：
干扰素诱导跨膜蛋白 (IFITM) 的广谱抗病毒活性主要归因于体外抑制病毒进入。在这里，我们使用基于禽肉瘤白血病病毒 (RCAS) 的基因转移系统，并成功生成了组成型表达鸡 IFITM3 (chIFITM3) 的雏鸡。 chIFITM3 过表达雏鸡对高致病性禽流感病毒 (HPAIV) H5N1（进化枝 2.2.1.2）表现出显着的保护作用和疾病耐受性。与对照雏鸡和攻击雏鸡相比，过表达chIFITM3的雏鸡还表现出临床症状的延迟发作、病毒脱落的减少以及组织病理学改变的减轻。这些发现强调，chIFITM3 的过度表达可在体内提供针对人畜共患 H5N1 的实质性防御。版权所有 © 2023 Institut Pasteur。由 Elsevier Masson SAS 出版。版权所有。
Broad-spectrum antiviral activities of interferon-induced transmembrane proteins (IFITMs) are primarily attributed to in vitro inhibition of viral entry. Here, we used an avian sarcoma-leukosis virus (RCAS)-based gene transfer system and successfully generated chicks that constitutively express chicken IFITM3 (chIFITM3). The chIFITM3-overexpressing chicks showed significant protection and disease tolerance against highly pathogenic avian influenza virus (HPAIV) H5N1 (Clade 2.2.1.2). The chicks, overexpressing chIFITM3, also showed delayed onset of clinical symptoms, reduced viral shedding, and alleviated histopathologic alterations compared to control and challenged chicks. These findings highlight that overexpression of chIFITM3 provide a substantial defense against zoonotic H5N1 in vivo.Copyright © 2023 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.