Armoracia rusticana P. G. Gaertner。属于十字花科，因其抗炎和抗癌活性而引起了科学界的兴趣。在一项持续研究中，我们发现了黄花菜的生物活性成分，从该植物的甲醇提取物中分离出了 19 种酚苷，包括三种未描述的黄酮醇糖苷和一种未描述的新木脂素糖苷。基于NMR光谱分析（1H、13C、1H-1H COSY、HSQC和HMBC）、HRESIMS和化学方法阐明了它们的结构。通过 ECD 和 LC-MS 分析确定了它们的绝对构型。通过在 C6 神经胶质瘤细胞系中诱导神经生长因子来评估所有化合物的潜在神经营养活性，并根据一氧化氮产生和促炎细胞因子（即 IL-1β）的抑制水平来评估其抗神经炎症活性。 、IL-6 和 TNF-α）在脂多糖激活的小胶质细胞 BV-2 细胞中。版权所有 © 2023 Elsevier Ltd。保留所有权利。

Armoracia rusticana P. G. Gaertner. belongs to the Brassicaceae family and has aroused scientific interest for its anti-inflammatory and anticancer activities. In a continuing investigation to discover bioactive constituents from A. rusticana, we isolated 19 phenolic glycosides including three undescribed flavonol glycosides and one undescribed neolignan glycoside from MeOH extract of this plant. Their structures were elucidated based on NMR spectroscopic analysis (1H, 13C, 1H-1H COSY, HSQC, and HMBC), HRESIMS, and chemical methods. The determination of their absolute configuration was accomplished by ECD and LC-MS analysis. All the compounds were assessed for their potential neurotrophic activity through induction of nerve growth factor in C6 glioma cell lines and for their anti-neuroinflammatory activity based on the measurement of inhibition levels of nitric oxide production and pro-inflammatory cytokines (i.e., IL-1β, IL-6, and TNF-α) in lipopolysaccharide-activated microglia BV-2 cells.Copyright © 2023 Elsevier Ltd. All rights reserved.