抑制细胞周期进程的天然产物可能具有作为抗癌剂的潜力。在本研究中，使用 HeLa/Fucci2 细胞通过荧光图像筛选微生物培养物提取物的细胞周期抑制作用。真菌 Akanthomyces sp. 的培养物提取物抑制了 S/G2/M 期的细胞周期进程，并且生物测定引导的提取物分级分离提供了三种先前未描述的 aphidicolin 衍生物，即 akanthomins A-C 和未描述的色酮糖苷，除了阿非迪霉素外，特别是 9-羟基丁香素 9-O-β-d-(4-O-甲基)吡喃葡萄糖苷。通过光谱分析和化学衍生化阐明了这些化合物的化学结构。使用流式细胞仪，发现 akanthomin A 和 aphidicolin 可以抑制 S 期的细胞周期进程。版权所有 © 2023 Elsevier Ltd。保留所有权利。

Natural products that inhibit cell cycle progression may have potential as anticancer agents. In this study, cell cycle inhibition of microbial culture extracts was screened by fluorescent images using HeLa/Fucci2 cells. The culture extract of a fungus, Akanthomyces sp., inhibited the cell cycle progression at the S/G2/M phases, and bioassay-guided fractionation of the extract afforded three previously undescribed aphidicolin derivatives, namely akanthomins A-C, and an undescribed chromone glycoside, specifically 9-hydroxyeugenetin 9-O-β-d-(4-O-methyl)glucopyranoside, in addition to aphidicolin. The chemical structures of these compounds were elucidated by spectroscopic analysis and chemical derivatization. Using a flow cytometer, akanthomin A and aphidicolin were found to inhibit cell cycle progression at the S phase.Copyright © 2023 Elsevier Ltd. All rights reserved.