六种未描述的化合物，uvarirufols D 和 E、( )-uvarigraanol B、(-)-uvarigraanol E、6-乙酰氧基-5-羟基-7-甲氧基黄烷酮和cherrevenaphene D，以及十二种已知化合物，包括多氧化环己烯、类黄酮和木酚素是从紫玉树茎的甲醇提取物中分离出来的。通过光谱分析阐明了它们的结构，并使用电子圆二色性确定了绝对构型。对几种分离株的细胞毒性、抗结核和抗炎潜力进行了评估。 (-)-6-Acetylzeylenol 对结核分枝杆菌表现出中等的抑制活性，MIC 值为 47.10 μg/mL。 Cherrevenaathene D 表现出弱的抗分枝杆菌活性，并对 RAW 264.7 细胞中脂多糖诱导的一氧化氮 (NO) 产生具有有效的抑制作用 (EC50 = 8.54 μM)。 8-Hydroxy-5,7-dimethoxyflavanone 显示出中等水平的 NO 抑制作用（EC50 = 43.62 μM），且细胞毒性很小。多氧化环己烯和木酚素对 HCT 116 和 22Rv1 癌细胞没有活性 (IC50 > 100 μM)。版权所有 © 2023 Elsevier Ltd。保留所有权利。

Six undescribed compounds, uvarirufols D and E, (+)-uvarigranol B, (-)-uvarigranol E, 6-acetoxy-5-hydroxy-7-methoxyflavanone and cherrevenaphthalene D, along with twelve known compounds, including polyoxygenated cyclohexenes, flavonoids, and lignans, were isolated from the methanol extract of Uvaria rufa stems. Their structures were elucidated by spectroscopic analyses and the absolute configurations were determined using electronic circular dichroism. Several isolates were evaluated for cytotoxic, antitubercular and anti-inflammatory potentials. (-)-6-Acetylzeylenol showed moderate inhibitory activity against Mycobacterium tuberculosis, with MIC value of 47.10 μg/mL. Cherrevenaphthalene D exhibited weak antimycobacterial activity and potent inhibitory effect on lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 cells (EC50 = 8.54 μM). 8-Hydroxy-5,7-dimethoxyflavanone displayed moderate level of NO inhibition (EC50 = 43.62 μM) with little cytotoxicity. The polyoxygenated cyclohexenes and lignans were inactive against HCT 116 and 22Rv1 cancer cells (IC50 > 100 μM).Copyright © 2023 Elsevier Ltd. All rights reserved.