硒是一种众所周知的与健康相关的元素，与癌症化学预防、神经保护作用有关，对糖尿病和多种传染病等有益。它天然存在于一些食物中，但人类缺乏这种元素导致了富含这种元素的营养保健品、补充剂和功能性食品的生产。硒水平与健康之间存在 U 形联系，而有毒水平和必需水平之间的范围很窄，因此，应谨慎补充。组学方法已成为深入研究哺乳动物膳食硒反应的重要方法，可以更深入地了解该元素的代谢及其生物学作用。在这篇综述中，我们讨论了从工作流程到应用的组学方法，这些方法以前曾用于深入了解膳食硒的代谢。特别关注硒蛋白、血液和组织（例如大脑、生殖器官等）中的代谢组学反应以及对肠道微生物群及其代谢物谱的影响。因此，我们主要回顾了杂原子标记的蛋白质组学、金属组学、代谢组学和元分类学，通常与转录组学、基因组学和其他分子方法相结合。© 2023 作者。蛋白质组学由 Wiley-VCH GmbH 出版。

Selenium is a well-known health-relevant element related with cancer chemoprevention, neuroprotective roles, beneficial in diabetes, and in several infectious diseases, among others. It is naturally present in some foods, but deficiency in people led to the production of nutraceuticals, supplements, and functional food enriched in this element. There is a U-shaped link between selenium levels and health and a narrow range between toxic and essential levels, and thus, supplementation should be performed carefully. Omics methodologies have become valuable approaches to delve into the responses of dietary selenium in mammals that allowed a deeper knowledge about the metabolism of this element as well as its biological role. In this review, we discuss omics approaches from the workflows to their applications that has been previously used to deep insight into the metabolism of dietary selenium. There is a special focus on selenoproteins, metabolomics responses in blood and tissues (e.g., brain, reproductive organs, etc.) as well as the impact on gut microbiota and its metabolites profile. Thus, we mainly reviewed heteroatom-tagged proteomics, metallomics, metabolomics, and metataxonomics, usually combined with transcriptomics, genomics, and other molecular methods.© 2023 The Authors. Proteomics published by Wiley-VCH GmbH.